تاثیر سیر ‌(Allium sativum) و پیاز قرمز ‌(Allium cepa) بر نشانه‌های آسیب‌شناختی بیماری آنفلوانزای پرندگان در جوجه‌های گوشتی مواجهه‌یافته با ویروس آنفلوانزای تحت‌تیپ H9N2

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.

2 دانشیار گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.

3 دانش‌آموخته دکترای حرفه‌ای، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.

چکیده

ویروس آنفلوانزای پرندگان، تحت­تیپ  H9N2در ایران شیوع بالائی داشته و از سراسر جهان هم گزارش شده­ است. وقوع بیماری آنفلوانزا باعث خسارت سنگین اقتصادی در صنعت طیور می‌شود. در مطالعه حاضر نقش سیر (Allium sativum) و پیاز قرمز (Allium cepa) در کاهش شدت نشانه­های آسیب‌شناختی بیماری آنفلوانزای پرندگان ناشی از تحت­تیپ H9N2 در جوجه­های گوشتی، بررسی شد. بدین منظور ابتدا سمیت سیر و پیاز مورد بررسی قرار­گرفت. همچنین تعداد 72 قطعه جوجه­ء یک روزه نژاد گوشتی سویه­­ء راس به 9 گروه 8 قطعه­ای تقسیم ­شدند که شامل‌ 4 گروه درمانی، 1 گروه کنترل مثبت و 4 گروه مسمومیت بودند. سپس گروه‌های درمانی در 28 روزگی با ویروس مواجهه داده شدند. پرندگان گروه‌های مواجهه داده شده یک روز بعد از مواجهه به مدت هفت روز، به‌طور جداگانه سیر و پیاز قرمز را همراه غذا دریافت کردند. در ادامه علائم بالینی، نشانه­های آسیب‌شناختی و نیز تیتر آنتی­بادی اختصاصی ضد ویروس آنفلوانزای تحت­تیپ H9N2 در سرم پرندگان مورد آزمایش بررسی شد. سیر و پیاز هیچ اثر سمی بر جوجه‌ها نداشتند. در آسیب‌شناسی روده نیز میزان افزایش سلول‌های جامی‌شکل و قطر پرزها در گروه‌های کنترل منفی و درمانی در مقایسه با گروه کنترل مثبت، بیشتر بود که از نظر آماری نیز اختلاف معنی‌دار داشتند (05/0p<). به نظر می­رسد سیر و پیاز قرمز که موجب کاهش شدت علائم آسیب‌شناختی بیماری آنفلوانزای پرندگان ناشی از تحت­تیپ H9N2 ویروس آنفلوانزا در دئودنوم جوجه­های گوشتی مورد مطالعه شدند، احتمالاً با کاهش تعداد ویروس‌ها، در ترمیم مخاط روده نقش دارند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of garlic (Allium sativum) and red onion (Allium cepa) on the histopathological signs of avian influenza disease in broiler chickens exposed to avian influenza virus subtype H9N2

نویسندگان [English]

  • Zolfaghar Rajabi 1
  • Amir Ali Shabazfar 2
  • Sadegh Haghparast kisami 3
1 Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
2 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
3 D.V.M. Graduate, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
چکیده [English]

Avian Influenza (AI) virus subtype H9N2 is endemic in Iran and reported from all over the world. It causes economic losses to the poultry industry. In this study the role of garlic (Allium sativum) and red onion (Allium cepa) in reducing the severity of the histopathological signs of the avian influenza disease caused by subtype H9N2 in broiler chickens was evaluated. Toxicity of garlic and onion was evaluated first, and then the treatment groups consisting of 72 one day old Ross broiler chicks divided into 9 treatment groups were challenged with the H9N2 virus at 28 days. After one day, the challenged groups received garlic and red onion with feed for seven days. During the study, antibody titers against the H9N2 virus, clinical signs, and histopathological samples were recorded. The garlic and onion had no toxic effect on chickens.  In histopathology, the percentage of goblet cells and villus diameter was statistically higher in negative control and treatment groups compared to the positive control group (p<0.05). It seems the garlic and red onion reduced the severity of the histopathological signs of the AI disease caused by subtype H9N2 in the duodenum of the broiler chickens. They are likely to play a role in repairing intestinal mucosa, by reducing the number of the viruses.

کلیدواژه‌ها [English]

  • chickens
  • Garlic
  • Histopathology
  • Influenza
  • onion
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