مطالعه تغییرات ایجادشده در پروتئین‌های فاز حاد سرم، هموگرام و الگوی الکتروفورتیکی پروتئین‌های سرمی اسب متعاقب تغییر جیره غذایی از علوفه به علوفه مخلوط با کنسانتره

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 دانش‌آموخته دکترای تخصصی کلینیکال پاتولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

2 استاد گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

10.30495/jvcp.2021.1920959.1293

چکیده

علی­رغم مطالعات گسترده­ای که در زمینه ارتباط نوع جیره و تأثیر آن بر فاکتورهای بیوشیمیایی اسب صورت گرفته، اما اطلاعات کمی در مورد پروتئین­های سرم و پلاسمای اسب به دنبال تغییر جیره غذایی از نوع علوفه به علوفه مخلوط با کنسانتره وجود دارد. پروتئین‌های مرحله حاد گروهی از پروتئین‌های سرم هستند که غلظت آن‌ها در پاسخ به عفونت‌ها، التهاب، تروما و نئوپلازی افزایش می‌یابد. از این رو، در مطالعه حاضر به ارزیابی سطح سرمی نشان­گر­های زیستی سرم، فیبرینوژن و همچنین غلظت پروتئین کل به همراه ارزیابی رفتار تغذیه‌ای اسب‌ها در هنگام تغییر جیره غذایی پرداختیم. بدین منظور، دو نوع جیره غذایی، شامل علوفه (تیمار اول) و علوفه مخلوط با کنسانتره (تیمار دوم) برای هر اسب به مدت پانزده روز متوالی استفاده شد. شمارش کامل سلول های خون و همچنین اندازه‌گیری سطح سرمی فیبرینوژن، هاپتوگلوبین و آمیلوئید نوع A، قبل و بعد از تغییر رژیم غذایی اندازه­گیری شد. سطح فیبرینوژن، هاپتوگلوبین و آمیلوئید  Aسرم به طور معنی‌داری در اسبان گروه تیمار دوم در مقایسه با اسب­های گروه تیمار اول افزایش داشت (05/0>p). همچنین اختلاف آماری معنی‌داری در شمارش کامل سلول­های خون بر اثر تغییر جیره غذایی از علوفه به علوفه مخلوط با کنسانتره مشاهده شد (05/0>p). به نظر می­رسد تغییر در فلور طبیعی دستگاه گوارش اسب متعاقب تغییر جیره غذایی می‌تواند بدن حیوان را در برابر صدمات حاد التهابی و آسیب­زا محافظت کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of dietary change from forage to concentrate on acute phase protein, hologram and serum protein electrophoresis in Horse.

نویسندگان [English]

  • Rouhoullah Karampour 1
  • Mohammad Razi Jalali 2
  • mohammad rahim haji hajikalaei 2
  • Alireza Ghadrdan mashhadi 2
1 PhD of Clinical Pathology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده [English]

Introduction: Despite extensive studies on the relationship between diet type and its effect on horse biochemical factors, little is known about horse serum and plasma proteins following changes in diet from forage to concentrate. Acute stage proteins are a group of serum proteins whose concentration increases in response to infections, inflammation, trauma, and neoplasia.

Material & Methods: in this study, we evaluated the serum levels of serum biomarkers, fibrinogen as well as total protein concentration along with evaluating the nutritional behavior of horses when changing diets. Two different diets (forage (first treatment), mixed forage with concentrate (second treatment) were performed for each horse for fifteen consecutive days.Complete blood count analysis as well as serum levels of fibrinogen, haptoglobin and type A amyloid were measured before and after dietary changes.
Results and Discussion: Serum fibrinogen, haptoglobin and amyloid A levels increased significantly (p <0.05) in the second treatment group compared to forage and a significant change was observed in the complete blood count results in changing the diet from the first to the second treatment (p <0.05). This can be due to the type of nutrition or acute inflammation caused by a change in the normal flora of the horse's digestive tract in the diet.

Conclusion :These changes can protect horses' bodies from acute inflammatory and traumatic injuries.

کلیدواژه‌ها [English]

  • horse
  • Forage
  • concentrate
  • Acute phase protein
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Study of changes in acute phase proteins of serum, hemogram and electrophoretic pattern of horse serum proteins following change of diet from forage to forage mixed with concentrate.