شناسایی مولکولی آناپلاسما فاگوسیتوفیلوم (Anaplasma phagocytophilum) در گاوها و گوسفندان استان گیلان

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 استادیار بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

2 دانشیار بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

3 مربی بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

چکیده

گونه آناپلاسما فاگوسیتوفیلوم یکیاز باکتری‌های بیماری­زای نوپدید منتقله توسط کنه‌ها می‌باشد که باعث ایجاد بیماری مشترک بین انسان و بسیاری از حیوانات می‌گردد و به همین دلیل اهمیت آن در بهداشت عمومی جامعه قابل‌توجه می‌باشد. هدف از انجام مطالعه حاضر، شناسایی مولکولی گونه آناپلاسما فاگوسیتوفیلوم در گاوها و گوسفندان استان گیلان بود. بدین منظور در طول سال 1397، تعداد 200 نمونه خون از طریق رگ وداج گاوها و 200 نمونه خون از طریق رگ وداج گوسفندان، به‌طور تصادفی از نقاط مختلف استان گیلان جمع‌آوری گردید. جهت آزمایش ­PCR (polymerase chain reaction)، در ابتدا DNA استخراجی از نمونه‌های خونی، با جفت آغازگری که قطعه 1468 جفت بازی از ژن 16S­rRNA جنس آناپلاسما را تکثیر می‌کند، تکثیر داده­شد. 70 نمونه‌ مثبت اولیه گاوی  با استفاده از روش nested-PCR از نظر وجود گونه آناپلاسما فاگوسیتوفیلوم بررسی شدند و در نتیجه 66 نمونه (33 درصد)، مثبت تشخیص داده شد. همچنین 38 نمونه مثبت  اولیه گوسفندی با استفاده از روش nested-PCR، ازنظر وجود گونه آناپلاسما فاگوسیتوفیلوم بررسی شدند که 10 نمونه (5 درصد)، از این نظرمثبت تشخیص داده شدند. تحلیل مقایسه­ای درصد فراوانی آلودگی به آناپلاسما­فاگوسیتوفیلوم گاوها و گوسفندان مورد آزمایش، در ارتباط با فصول مختلف سال، سنین مختلف، جنسیت دام، ارتفاع از سطح دریا، ناقلین و نوع دامداری (نیمه‌صنعتی- سنتی) هم نشان داد که فقط فراوانی گونه آناپلاسما فاگوسیتوفیلوم در گوسفندان استان گیلان، بین دو نیمه اول و دوم سال دارای اختلاف آماری معنی‌دار بود (05/0 p < /em><). نتایج مطالعه نشان داد که آناپلاسما فاگوسیتوفیلوم از گونه­های رایج آناپلاسما در بین دام­های استان گیلان می­باشد. با توجه به نقش آناپلاسما فاگوسیتوفیلوم در بروز آناپلاسموزیس گرانولوسیتیک در انسان، این موضوع به لحاظ بهداشت عمومی حائز اهمیت می­باشد.  

کلیدواژه‌ها


عنوان مقاله [English]

Molecular identification of Anaplasma phagocytophilum in cattle and sheep of Gilan province

نویسندگان [English]

  • nasrollah Vahedi Nouri 1
  • Vahid Noaman 2
  • Ebrahim Rahimabadi 3
1 Assistant Professor, Parasitic Diseases Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
2 Associate Professor, Parasitic Diseases Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
3 Instructor, Parasitic Diseases Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
چکیده [English]

Anaplasma phagocytophilum is one of the emerging pathogenic bacteria transmitted by mites, which causes zoonotic disease, between humans and many animals. Hence, its importance in public health is significant. The aim of this study was molecular identification of Anaplasma phagocytophilum in cattle and sheep of Gilan province. For this purpose, during 2018, 200 blood samples of cattle, and 200 blood samples from sheep were collected randomly via the jugular vein from different parts of Gilan province. The extracted DNA from blood cells were amplified by Anaplasma-all primers, which amplify an approximately 1468bp DNA fragment from region of 16S rRNA gene from various members of the genus Anaplasma. All 70 bovine positive specimens wereanalyzed for the presence of A. phagocytophilum with proprietary nested-PCRand 66 of blood samples (33%) were positive for A. phagocytophilum. In addition, 38 sheeppositive samples with proprietary nested-PCR wereanalysed for the presence of A.phagocytophilum and 10 of sheep blood samples (5%) were positive for A. phagocytophilum. The chi-squaretest was used to compare the percentage of A.phagocytophilum contamination of cattle and sheep in association with different seasons, different ages, animal gender, sea level, carriers and type of animal husbandry (semi-industrial-traditional). The results of this comparison indicated that only the abundance of A. phagocytophilum in sheep of Gilan province had a significant difference between the first and second half of the year (p < /em><0.05). The results of this study showed that A.phagocytophilum is a common species of Anaplasma in livestock of Gilan province. Given the role of this species in the occurrence of granulocytic anaplasmosis in humans, this issue is important in terms of public health.

کلیدواژه‌ها [English]

  • Anaplasma phagocytophilum
  • Molecular identification
  • cattle
  • Sheep
  • Gilan
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