ارزیابی آلودگی به استرپتوکوکوس اکویی در اسب‌های کار به ظاهر سالم منطقه شهرستان ارومیه به روش الایزای غیرمستقیم

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.

2 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.

10.30495/jvcp.2020.1880960.1246

چکیده

گورم یکی از مهم­ترین بیماری‌های عفونی اسب‌سانان می‌باشد که توسط استرپتوکوکوس اکویی تحت­گونه اکویی ایجاد می‌شود. بیماری با تب، بی اشتهایی، بی­حالی، ترشحات چرکی بینی و درگیری عقده­های لنفی به­ ویژه در ناحیه سر و گردن مشخص می­شود. هدف از مطالعه حاضر تعیین فراوانی سرمی آلودگی به استرپتوکوکوس اکویی در اسب‌های کار شهرستان ارومیه واقع در شمال غرب ایران بود. نمونه‌های سرمی از 46 رأس اسب به ظاهر سالم اخذ و به روش الایزای غیرمستقیم برای ردیابی ایمونوگلبولین جی ضد پادگن  SeM (Streptococcusequi M protein) آزمایش شد. همچنین داده‌ها برای تعیین اثر سن و جنس روی فراوانی موارد مثبت تجزیه و تحلیل شد. در کل 9/73 درصد (فاصله اطمینان 95 درصد: 6/86-2/61) از نمونه‌هابرای پادتن­های استرپتوکوکوس اکویی مثبت بودند. فراوانی سرمی تحت تاثیر سن (05/0<p < /em>) و جنس (05/0<p < /em>) قرار نداشت. در آنالیز رگرسیون از میان دو متغیر پیش‌بینی کننده سن و جنس، تنها سن معنی‌دار بود (05/0>p < /em>). یافته­های مطالعه حاضر مشخص کرد که با افزایش یک ‌سال سن احتمال آلودگی 403/1 برابر (فاصله اطمینان 95 درصد: 958/1-006/1) افزایش می‌یابدو سن 339/0 درصد از تغییرات آلودگی را توجیه می‌کند. جمع­بندی نهائی نتایج نشان داد که مواجهه بسیار بالا با استرپتوکوکوس اکویی در اسب‌های کار شهرستان ارومیه وجود دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of Streptococcus equi infection in apparently healthy working horses of Urmia region by indirect ELISA method

نویسندگان [English]

  • Esmail Minai 1
  • Arash Araghi-Sooreh 2
1 Gratuate of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 Associate Professor, Department of Clinical Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran.
چکیده [English]

Strangles is one of the most important infectious diseases of equids caused by Streptococcus equi subspecies equi. The disease is characterized by fever, anorexia, lethargy, purulent nasal discharge, and lymphadenopathy, particularly of the lymph nodes of the head and neck. The objective of this study was to determine the seroprevalence rate of S. equi infection in apparently healthy working horses of Urmia region in northwestern Iran. Sera from 46 apparently healthy horses were tested by enzyme linked immunosrobent assay (ELISA) kit (ID Screen S. equi Indirect) to detect IgG to S. equi M protein (SeM) antigen. Data were analyzed for effect of sex and age on seropositivity by chi-squared, fisher’s exact and logistic regression tests. A total of 73.9% (95% CI: 61.2-86.6%) of samples were positive for S. equi antibodies. Seropositivity was not affected by age (p < /em>>0.05) and sex (p < /em>>0.05). In regression analysis only age was significant between predictor variables. Odds of infection between the age based on year and disease was 1.403 (95% CI: 1.006-1.958), and age explained 0.339% of infection’s fluctuations. The final summary of results indicates that exposure to S. equi is very high in apparently healthy working horses of Urmia region.

کلیدواژه‌ها [English]

  • Streptococcus equi
  • horse
  • Elisa
  • Urmia
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