مطالعه اثر عصاره فلفل کوهی (Vitagnus castus) بر بلوغ و لقاح آزمایشگاهی اووسیت موش‌های سوری مبتلاشده به سندروم تخمدان پلی‌کیستیک

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی فیزیولوژی دام، دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.

2 استادیار گروه علوم دامی دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.

3 دانشیار گروه علوم آناتومی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران.

4 استادیار گروه علوم طیور، دانشکدة کشاورزی، دانشگاه تربیت مدرس، تهران، ایران.

10.30495/jvcp.2020.1881036.1245

چکیده

عدم کاهش کلاژن دیواره فولیکولی منجر به تجمع فولیکول­ها و بروز سندرم تخمدان پلی­کیستیک و کاهش کیفیت اووسیت­ها میگردد. هدف ازمطالعه حاضر بررسی اثر عصاره گیاه فلفل­کوهی (Vitagnus castus) بر بلوغ و توان باروری آزمایشگاهی اووسیتها در موش­های سوری مبتلاشده به سندرم تخمدان پلی­کیستیک (ovarian syndrome; PCOpolycystic) بود. بدین منظور تعداد 32 سر موش سوری ماده NMRI (Naval Medical Research Institute) ماده نابالغ 25 روزه با میانگین وزنی 25 گرم تهیه­شده از دانشگاه تبریز، به­طور تصادفیبه 4 گروه آزمایشی به شرح زیر تقسیم شدند: گروه شاهد بدون دریافت عصاره، گروه کنترل PCO بدون دریافت عصاره،  گروه PCO دریافت­کننده365 میلی­گرم عصاره فلفل­کوهی و گروه PCO دریافت­کننده730میلی­گرم عصاره فلفل‌کوهی. برای تهیه اووسیت، موش­های آزمایشی توسط تزریق درون صفاتی هورمون PMSG ­(pregnant mare serum gonadotropin) تیمار شدند و بعد از آسان­کشی،از فولیکول­های تخمدانی تخمک­گیری به­عمل آمده و برای ارزیابی بلوغ و لقاح آزمایشگاهی مورد استفاده قرار گرفتند.نتایج نشان داد که مقادیر هورمون­های تستوسترون و استروژن در گروه­های PCO تیمارشده با عصاره فلفل­کوهی نسبت به گروه ­PCOدریافت­کننده استرادیول ­والرات به طور معنی­داری پایین­تر بود (05/0>p < /em>).همچنین تعداد اووسیت­های نابالغی که به مرحله متافازII رسیدند در گروهPCOدریافت­کننده730 میلی­گرم عصاره فلفل­کوهی نسبت به گروه PCO دریافت­کننده استرادیول­والرات به طور معنی­داری بالاتربود (05/0>p < /em>). اما درصد اووسیت­های لقاح یافته و نیز بلاستوسیست­های تولیدی بین گروه­های آزمایشی تفاوت معنی­داری با هم نداشتند (05/0<p < /em>). از طرف دیگر درصد رویان­های هچ­شده در گروه  PCO­ دریافت کننده365 میلی­گرم عصاره فلفل­کوهی به طور معنی­داری بالاتر از گروه ­PCOدریافت­کننده استرادیول­والرات بود (05/0>p < /em>). یافته­های پژوهش حاضر نشان داد که عصاره گیاه فلفل کوهی باعث تکامل بیشتر اووسیت­ها جهت لقاح و نیز افزایش تکامل رویان­های حاصل از لقاح آزمایشگاهی در حیوانات درگیربا سندرم تخمدان پلی­کیستیک می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Therapeutic effects of Vitex (Vitagnus castus) extract on in vitro maturation and fertilization of oocytes in mice affected by polycystic ovary syndrome

نویسندگان [English]

  • tohid ghorbani 1
  • amir karimi 2
  • gholamreza najafi 3
  • maghsud besharti 2
  • M. Sharafi 4
1 MSc Student, Department of Animal Science, Faculty of Ahar Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.
2 Assistant Professor, Department of Animal Science, Faculty of Ahar Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.
3 Associated Professor, Department of Anatomy, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
4 Assistant Professor, Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran.
چکیده [English]

Polycystic ovary syndrome results from lack of follicular wall collagen reduction leading to accumulation of follicles and decreased oocyte quality. This study aimed to evaluate the effect of vitex extract on in vitro maturation and fertilization of oocytes in mice exposed to polycystic ovary syndrome (PCOs). A total of 32 immature Naval Medical Research Institute (NMRI) 25 day old female mice with a mean body weight of 25 gr were randomly allocated into four experimental groups including: 1) Control group (Con) which did not receive extract, 2) PCO: polycystic ovary syndrome group which also did not receive any extract, and groups 3 and 4 in which PCO was induced and received vitex extract at 365 and 730 mg/kg/day respectively for 30 days.  The mice received intraperitoneal injection of pregnant mare serum gonadotropin (PMSG) and subsequently sacrificed in order to collect oocytes from ovarian follicles for in vitro analysis of maturation and fertilization. The amounts of serum testosterone and estradiol substantially decreased in extract treated groups in comparison with PCO mice (p < /em><0.05). Use of vitex extract decreased testosterone whether in groups PCO+365 mg of extract or PCO+730 mg of extract versus PCO (p < /em><0.05). The number of mature oocytes (MII) in PCO+730 mg of extract was significantly higher than PCO (p < /em><0.05). Also there were no significant differences in percentage of fertilized oocytes and Two-cell embryos between experimental groups (p < /em>>0.05). On the other hand, the percentage of produced blastocyst and hatched embryos in PCO+365 mg of extract was higher than PCO (p < /em><0.05). The results showed vitex extract consumption can induce more oocytes for fertilization and consequently, production of embryos in animals with PCO syndrome.

کلیدواژه‌ها [English]

  • PCOs
  • Vitex
  • In vitro maturation
  • in vitro fertilization
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