تاثیر عصاره هیدرو‌الکلی گیاه بابونه‌ رومی (Anthemis nobilis L.) بر پارامتر‌های تولیدمثلی و رفتاری موش‌های صحرایی نر تحت استرس عدم ‌تحرک

نوع مقاله: علمی پژوهشی

نویسندگان

1 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

2 باشگاه پژوهشگران جوان و نخبگان، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

3 استادیار گروه علوم پایه، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

4 دانشیار گروه پاتوبیولوژی، دانشکده پیرا‌دامپزشکی، دانشگاه ایلام، ایلام، ایران.

5 دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

چکیده

استرس عامل بسیار مهمی در ایجاد اختلالات در دستگاه تولید­مثل می­باشد. با توجه به اثرات بابونه­­ رومی (Anthemis nobilis L.) در طب سنتی بر برخی از بیماری­ها، هـدف از انجام مطالعه حاضر، بررسی تاثیر عصاره هیدرو‌­الکلی بابونه ­رومی بر پارامتر­های تولید­مثلی و رفتاری موش­های صحرایی نر تحت استرس عدم تحرک بود. تعداد 30 سر موش صحرائی نر بالغ نژاد ویستار بـه طـور تصـادفی در پنج گـروه شامل گروه­های شم سالم، شاهد تحت استرس و سه گروه تیمار تحت استرس  با دریافت‌ دوزهـای 50، 100 و 200 میلـی‌گـرم بـر کیلـوگرم وزن بدن از عصـاره هیدرو الکلـی بابونه ­­رومی تقسیم و به مدت 21 روز نگه­داری شدند. در پایان دوره جهت سنجش میزان استرس از آزمون ماز به­علاوه­ای شکل مرتفع استفاده شد. همچنین به منظور تعیین غلظت هورمون­های تستوسترون،LH  (luteinizing hormone) و FSH (follicle-stimulating hormone)­، نمونه­ خون از همه موش­ها اخذ شد. در ادامه نیز تعداد اسپرم­های اپیدیدیمی بررسی گردید. نتایج به­دست آمده با روش آماری آنالیز واریانس یک طرفه و هچنین آزمون‌ مقایسه‌ای چند‌گانه توکی تحلیل گردید. عصاره هیدروالکلی گیاه بابونه منجر به افزایش معنی­دار تعداد اسپرم و هورمون­های تستوسترون، LH و FSH  نسبت به گروه شاهد استرس شد (05/0>p). همچنین عصاره مذکور سبب افزایش معنی­دار درصد ورود به بازوی باز و کاهش درصد ورود به بازوی بسته ماز به­علاوه­ای شکل مرتفع در مقایسه با گروه شاهد استرس گردید (05/0>p). در مطالعه حاضر عصاره هیدرو‌الکی بابونه رومی اثرات ضد­ ­اضطرابی داشته و موجب افزایش تعداد اسپرم، میزان تستوسترون، LH و FSHشد که احتمالاً این افزایش در ارتباط با ترکیبات آنتی­اکسیدانی این گیاه می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Anthemis nobilis L. hydro alcoholic extract on parameters of reproductive and behavioural in male rats exposed to immobility stress

نویسندگان [English]

  • M Neshat Gharamaleky 1
  • yasin bagheri 2
  • masoud delashoub 3
  • hesam rafie 2
  • ali mohammad bahrami 4
  • aref delkhosh 5
1 Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2 Young Researchers and Elite Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 Assistant Professor, Department of Basic Science, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
4 Associate Professor, Department of Pathobiology, Para Veterinary Faculty of Ilam, University of Ilam, Ilam, Iran.
5 D.V.M. Graduate, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
چکیده [English]

Introduction: Nowadays the life style and conditions have caused that human exposed to various stresses. Various types of physiological and psychological stresses affect the hypothalamic-pituitary-adrenal cortex, the hypothalamic-pituitary-gonadal axis, the adrenomodular sympathetic system, and the sympathetic nervous system, resulting in alterations in some of the body organs. One of the most affected systems is reproductive organs and this effect is triggered by activating a cascade of reactions. There are reports that stress with various degrees resulted to reduced testosterone secretion and may affect spermatogenesis in males. One of the strongest of stress is immobility, which results in an increase in some hormones such as adrenaline, noradrenaline and corticosterone. According to research, this type of stress in the brain increases nitric oxide production, which suppresses testosterone production. Now, medicinal plants and their derivatives account for 20 percent of prescriptions in advanced industrialized countries and 80 percent in developing countries. Anthemis nobilis L. is one of the native plants of Mediterranean region which is used in traditional Iranian medicine as a pain reliever and antispasmodic agent. Previous researches have shown that Anthemis nobilis L. can have positive effects on reproductive parameters. Considering the effects of Anthemis nobilis L. in traditional medicine as an effective medicine on some diseases, the aim of this study is evaluating the effect of hydro-alcoholic extract of Anthemis nobilis L. on parameters of reproductive and behavioural in male rats exposed to immobility stress.
Materials and Methods: 30 male rates weighing 220 ± 20 g were randomly divided into five group include: sham without any stress and additive, control exposed to stress without additive, treatment groups exposed to stress and received of Anthemis nobilis L. in dosage 50, 100 and 200 mg/kg respectively. The extract was given to the mice with gavage and accompanied by immobility stress for 21 consequence days. At the end of study, anxiety was evaluated using elevated plus maze, also blood samples were collected for hormones assay and number of sperms were assessed. All procedures of elevated plus maze recorded by a digital camera and behaviors evaluated using Panlab v.3 software. Obtained data were analyzed using SPSS v.23 software under windows and to compare differences between the groups, one-way ANOVA and Tukey post-hoc test was used.
Results and discussion: Studies showed that 50, 100 and 200 mg/kg hydro-alcoholic extract of Anthemis nobilis L. could increase the number of sperm (respectively 51.32±2.61, 54.25±3.08 and 58.11±3.16) , Testosterone (respectively 2.24±0.10, 2.40±0.09 and 2.64±0.13), LH (respectively 1.83±0.10, 1.96±0.13 and 2.1±0.12) and FSH (respectively 3.58±0.17, 3.72±0.11 and 4.12±0.12) levels compared to the stress group significantly (p

کلیدواژه‌ها [English]

  • Anthemis nobilis L
  • immobility stress
  • sperm
  • Rat
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