اثر سولفات منیزیم بر استرس اکسیداتیو القا شده توسط لتروزول در بافت تخمدانی موش‌های صحرایی ماده بالغ نژاد ویستار

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه زیست شناسی ، دانشکده علوم پایه، واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی ، تهران

2 گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 دانشگاه آزاد إسلامی واحد علوم و تحقیقات

10.30495/jvcp.2020.561604.1182

چکیده

منیزیم دومین عنصر فراوان بعد از پتاسیم در سلول می‌باشد و نقش مهمی در عملکردهای گوناگون بیولوژیکی ایفا می‌کند. سندرم تخمدان پلی‌کیستیک (Polycystic ovary syndrome, PCOS) یک بیماری شایع غدد درون‌ریز در زنان در سنین باروری می‌باشد که اغلب با سندرم متابولیک همراه است. شواهد نشان می‌دهند که استرس اکسیداتیو و درجات پایین التهاب مزمن نقش مهمی را در پاتوژنز PCOS ایفا می‌کنند. هدف از مطالعه حاضر، بررسی اثر سولفات منیزیم بر استرس اکسیداتیو القاء‌شده توسط لتروزول در بافت تخمدان موش‌های صحرایی ماده بالغ نژاد ویستار بود. بدین منظور تعداد 36 سر موش صحرایی ماده به صورت تصادفی به 6 گروه 6 تایی شامل کنترل سالم (بدون تیمار)، تیمار سالم (تیمار خوراکی سولفات منیزیم در دوز 100 میلی‌گرم برکیلوگرم وزن بدن)، کنترل آسیب تخمدانی (تیمار خوراکی لتروزول به میزان 1 میلی‌گرم بر کیلوگرم وزن بدن ) و تیمار تجربی آسیب تخمدانی (تیمار با سولفات منیزیم در دوزهای 10، 50 و 100 میلی‌گرم بر ‌کیلوگرم وزن بدن همراه لتروزول) تقسیم شدند. حیوانات 24 ساعت پس از آخرین دوز درمان در روز 31 آسان‌کشی شده و فعالیت آنزیم‌های سوپراکسید دیسموتاز (Superoxide dismutase, SOD)، کاتالاز (Catalase, CAT) و گلوتاتیون پراکسیداز (Glutathione peroxidase, GPX) در بافت تخمدان آن‌ها بررسی گردید. تیمار سولفات منیزیم باعث افزایش معنی‌دار میزان فعالیت آنزیم‌های SOD،CAT و GPX بافت تخمدان نسبت به گروه کنترل شد (05/0p

کلیدواژه‌ها


عنوان مقاله [English]

Effect of magnesium sulfate on letrozole-induced oxidative stress in ovarian tissue of adult female Wistar rats

نویسندگان [English]

  • Zahra Aslahnezhad 1
  • Akram Eidi 2
  • Pejman Mortazavi 3
  • Shahrbanoo Oryan 2
1 Department of Biology, basic sciences, Science and Research Branch, Islamic Azad University, Tehran
2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Science and Research Branch, Islamic Azad University, Tehran
چکیده [English]

Background and Purpose: Magnesium is the fourth most abundant cation and the second most abundant intracellular cation in the human body. Magnesium is involved in many essential physiological functions. It is a co-factor for over 300 enzymatic reactions, many of which involve generation of adenosine triphosphate (ATP), it regulates transmembrane transport of other ions, including calcium and potassium, and stabilizes secondary structures of DNA and RNA. Consequently, magnesium is essential for muscle contraction and relaxation, cardiac rhythm, vascular tone, neurological function, and cell proliferation. Magnesium is required for cell proliferation, cellular energy production, mineral metabolism, bone development, and glucose homeostasis. Nutrition surveys in North America indicate that magnesium consumption is below recommended intakes for a large segment of the population. Furthermore, diseases such as type 2 diabetes and use of certain medications can increase magnesium loss and predispose individuals to magnesium deficiency. The low magnesium intakes in comparison to current recommendations combined with the high prevalence of factors that can increase magnesium requirements raise a concern about widespread Mg deficiency. Biochemical data lend further support. Hypomagnesemia exists in the general population and the incidence is high in certain subpopulations. Since magnesium is required for many enzymatic reactions, Magnesium deficiency can presumably affect numerous physiological processes. Some studies have reported changes in body composition with dietary magnesium restriction. In rats, maternal and postnatal feeding of a magnesium-deficient diet decreased body weight, lean body mass, and fat-free mass and increased percentage body fat in the offspring. In contrast, body weight, fat mass, and lean mass were similar in rats fed a high-fat diet containing normal or low magnesium beginning after weaning. Polycystic ovary syndrome (PCOS) is a prevalent endocrinological disorder in reproductive-age women and is often associated with metabolic syndrome. Evidence suggests that oxidative stress and low degrees of chronic inflammation play an important role in the pathogenesis of PCOS. PCOS is the most common endocrine disorder in premenopausal women. PCOS is a common and multifactorial disease that affects approximately 4-18% of all reproductive-aged women in the world. In the clinic, hyperandrogenism and insulin resistance appear to be the major etiological drivers for reproductive and metabolic abnormalities in women with PCOS. While it is believed that anovulation is the main reason for infertility in PCOS patients, accumulating evidence from clinical studies also indicates that the impairment of endometrial function likely causes recurrent pregnancy loss, premature delivery, endometrial hyperplasia, and carcinoma in women with PCOS. Additionally, several lines of evidence suggest that the systemic low-grade inflammation that often coincides with PCOS compromises multiple aspects of fertility. A deficiency in the activity of aromatase was one reasonable intraovarian disturbance in steroidogenesis thought to cause PCOS. Because aromatase catalyzes the rate-limiting step in the biosynthesis of oestrogens from androgens, a decrease in the activity of this enzyme could be expected to result in increased ovarian androgen production and development of PCOS. The purpose of this study, the effect of magnesium sulfate on letrozole-induced oxidative stress was investigated in ovarian tissue of adult female Wistar rats.
Materials and Methods: In this study, a total of 36 female rats were randomly divided into 6 groups of 6: The normal control group (intact), the healthy experimental group (magnesium sulfate 100 mg/kg body weight, gavage), ovarian damage control group (letrozole 1 mg/kg body weight, gavage), ovarian damage experimental group (magnesium sulfate 10, 50, and 100 mg/kg body weight together letrozole). The animals were euthanized 24 h after the last dose of the treatment on day 31. Ovaries were immediately obtained after the animals were sacrificed. The ovaries were homogenized and centrifuged. The supernatant was used to assay the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in ovarian tissue were investigated. Data were analyzed using one-way ANOVA and Tukey test. The criterion was significant (p

کلیدواژه‌ها [English]

  • magnesium sulfate
  • Letrozole
  • PCOs
  • antioxidants
  • Rat