بررسی تغییرات هموگرام، شکنندگی اسمزی و آنزیم‌های آنتی‌اکسیدان گلبول‌های قرمز در سگ‌های تحت درمان با سیر

نوع مقاله: علمی پژوهشی

نویسندگان

1 استاد گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

2 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

3 دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

چکیده

سیر به عنوان یک گیاه دارویی با خواص آنتی­اکسیدانی، ضد هیپرلیپیدمی و ضد دیابتی، به­طور گسترده­ای در درمان بیماری­ها، مورد استفاده قرار می­گیرد. هدف از انجام مطالعه حاضر، بررسی عوارض احتمالی هماتولوژیک و نیز فعالیت برخی آنزیم­های آنتی‌اکسیدان گلبول­های قرمز متعاقب تجویز سیر در سگ­ بود. در این مطالعه 10 قلاده سگ نر، تحت درمان با قرص سیر با دوز mg/kg 100، روزانه یک­بار و برای مدت 45 روز قرار گرفتند. خون‌گیری از سگ­ها در سه نوبت طی روز­های صفر، 45 و 60 مطالعه صورت گرفت. شمارش سلول‌های خونی، بررسی مورفولوژی گلبول­های قرمز، شمارش رتیکولوسیت­های خون، اجسام هینز و نیز آزمایش شکنندگی اسمزی گلبول­های قرمز انجام گرفت. همچنین فعالیت آنزیم­های آنتی­اکسیدان گلبول­های قرمز شامل سوپراکسید دیسموتاز (superoxide dismutase; SOD) و گلوتاتیون پراکسیداز (glutathione peroxidase; GPX) سنجیده شد. تجویز سیر در سگ­ها، منجر به کاهش معنی­دار در تعداد گلبول­های قرمز، هماتوکریت و میزان هموگلوبین در روز 60 نسبت به روز صفر مطالعه گردید (05/0p<). همچنین میانگین هموگلوبین گلبولی (mean corpuscular hemoglobin; MCH) در روزهای  45و60 و میانگین غلظت هموگلوبین گلبولی (mean corpuscular haemoglobin concentration; MCHC) در روز60، به­طور معنی­داری کاهش یافته بود (05/0p<). در بررسی شکنندگی اسمزی گلبول­های قرمز، درصد همولیز در غلظت­های نمک 55/0، 50/0، 45/0 و 40/0 درصد، به­طور معنی­داری کاهش یافته بود (05/0p<). همچنین افزایش معنی­داری در میزان فعالیت آنزیم­های آنتی­اکسیدان در گلبول­های قرمز، در روز 60 مشاهده گردید (05/0p<). مطالعه حاضر نشان داد که تجویز قرص سیر با دوز استفاده شده در مطالعه حاضر­، اثرات تخریبی بر گلبول‌های قرمز ایجاد نکرده و منجر به بهبود سیستم دفاع آنتی­اکسیدانی و مقاومت اسمزی این سلول­ها شده است.

کلیدواژه‌ها


عنوان مقاله [English]

A survey on hemogram, osmotic fragility and antioxidant enzyme changes of erythrocytes in dogs treated with Garlic

نویسندگان [English]

  • Bahman Mosallanejad 1
  • Misagh Jalali 2
  • Mohammad Razi Jalali 1
  • Shahrzad Alipoor 3
1 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده [English]

Introduction: The vital properties of garlic have been known since ancient times. It is an herbal medicine with antioxidant, anti-hyperlipidemic and antidiabetic properties which is extensively used in the treatment of diseases. Garlic is rich in sulfur compounds, mainly in the form of cysteine derivatives. Among domestic animals, dogs and cats are more sensitive to the oxidative effects of Allium sativum family plants including garlic and onion due to differences in antioxidant defense system of red blood cells (Kovalkovicova et al., 2009). Herbal remedies are usually cheaper than chemical medicines. However, some reports suggest that poisoning and hematological complications may occur following the consumption of raw garlic or its extract in dogs (Lee et al., 2000). The purpose of the present survey was to assess probable hematologic alterations and the activity of some erythrocyte antioxidant enzymes following garlic administration in dogs.
Material and Methods: The present study was performed in Veterinary Hospital of Shahid Chamran University of Ahvaz during October-March 2015. For this purpose ten mixed breed dogs were selected with an average age of 1 to 2 years old in a similar weight range (19.6 kg). All the studied dogs were clinically healthy. They were selected from the population of rural dogs around Ahvaz district. Age of dogs was estimated according to dental formulary and tooth wear. In this research, all dogs were treated with garlic tablet (100 mg/kg, only once daily and for 45 days). Blood samples were collected three times on days zero, 45 and 60 of challenge. Complete blood count (CBC), erythrocyte morphology, reticulocyte count, Heinz body and erythrocyte osmotic fragility test were measured in different times. Evaluation of antioxidant enzymes activity of red blood cells including superoxide dismutase (SOD) and glutathione peroxidase (GPX) were performed by commercial kits (Randox, UK) and spectrophotometric method (Optima 3000 plus, Japan). According to the kit instruction, 0.5 ml of whole blood was slipped after three steps of washing with normal saline (NaCl 0.9%) (Merck, Germany), by adding 2 ml of cold distilled water (4° C). The obtained samples were stored at -70° C until testing.
Results and Discussion: It was determined that the use of garlic tablet with dosage of 100 mg/kg was tolerated in the studied dogs. No clinical complications were observed in animals (such as diarrhea, vomiting and anorexia). Garlic administration caused a significant reduction in erythrocyte count, hematocrit and hemoglobin concentration on day 60 compared to zero day (p

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Dog
  • Garlic
  • Hemogram
  • Osmotic fragility
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