بررسی اثر نانوذره سلنیم و سلنیت سدیم بر بیان ژن ادیپونکتین در جفت میش‌های آبستن

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانش‌آموخته دکترای تخصصی بیماری‌های داخلی دام‌های بزرگ، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.

2 استاد گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.

3 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.

4 استادیار گروه ژنتیک، دانشکده علوم، دانشگاه شهرکرد، شهرکرد، ایران.

5 کارشناس پرستاری، واحد آستارا، دانشگاه آزاد اسلامی، آستارا، ایران.

چکیده

در سال‌های اخیر توجهی ویژه به واسطه‌های مقاومت انسولینی نظیر ادیپونکتین شده است که در دوران آبستنی در بافت جفت تولید و بیان شده و از این طریق اثرات خود را بر جنین در حال رشد و سلامت مادر القاء می­کنند. در این مطالعه برای اولین بار، تأثیر نانوذره سلنیم و سلنیت سدیم بر میزان نسخه‌برداری ژن ادیپونکتین بافت جفت در دوره انتقالی مورد مطالعه قرار گرفت. به این منظور، 20 رأس میش 4 ماهه آبستن، به‌صورت تصادفی انتخاب و در زمان 10 روز منتهی به زایمان، هر روز تجویز خوراکی مکمل‌های سلنیت سدیم (به میزان 1/0 میلی‌گرم به ازای هر کیلوگرم وزن بدن) و نانوذره سلنیوم (در دو دوز 05/0 و 1/0 میلی‌گرم به­ازای هرکیلوگرم وزن بدن) به آن­ها صورت گرفت. به گروه شاهد هم آب مقطر با حجم مشابه خورانده شد. پس از زایمان با نمونه‌برداری از جفت، میزان نسخه‌برداری از ژن ادیپونکتین به روش real-time RT-PCR و بر اساس روش مقایسه‌ای ΔΔCt-2 تعیین شد. نتایج حاصله نشان داد که اضافه نمودن مکمل‌های سلنیومی به جیره غذایی میش‌های آبستن، موجب کاهش معنی‌دار در میزان نسخه‌برداری ژن ادیپونکتین می‌شود که در این خصوص، عملکرد سرکوب‌گری نانوذره سلنیوم به مراتب برتر از سلنیت سدیم بود.

کلیدواژه‌ها


عنوان مقاله [English]

Study of selenium nanoparticles and sodium selenite supplementation effects on expression of adiponectin gene in pregnant ewes

نویسندگان [English]

  • Pedram Moayeri 1
  • gholam ali kojouri 2
  • afshin jafari dehkordi 3
  • ali mohammad ahadi 4
  • mahsa abolfazlzadeh 5
1 Shahrekord UnivePh.D. Graduate of Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.rsity
2 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.
3 Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.
4 Assistant Professor, Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.
5 Graduate of Nursing, Astara Branch, Islamic Azad University, Astara, Iran.
چکیده [English]

In recent years, special attention has been paid to insulin resistance mediators, such as adiponectin, that is produced and expressed during conception in placenta tissue, thereby inducing its effects on the growing embryo and the health of the mother. In this study, for the first time, Twenty four-month pregnant ewes within the same age were selected randomly. During the 21 days leading up to birth, oral administration of selenium nanoparticles (Se NPs) with dosages of 0.05 and 0.10 mg/kg B.W. and sodium selenite with dosage of 0.1 mg/kg B.W. was carried out. At the same time the control group was fed distilled water in equal volume. With sampling of the placenta during childbirth, transcription amount of adiponectin gene was determined by RT PCR Real Time based on a comparison assay of 2-ΔΔCt. The results showed that the addition of selenium supplements to the diet of ewes of pregnant caused a significant decrease in the amount of adiponectin gene transcription, and the selenium nanoparticle suppression was significantly superior to sodium Selenite.

کلیدواژه‌ها [English]

  • Adiponectin
  • oral administration
  • pregnant ewes
  • selenium nanoparticles
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