بررسی میزان شیوع کریپتوسپوریدیوم و ژیاردیا در سگ‌های گله و نگهبان مناطق روستایی اطراف مهاباد

نوع مقاله: علمی پژوهشی

نویسندگان

1 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.

2 استادیار دانشکده دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران.

چکیده

تک­یاخته­های جنس کریپتوسپوریدیوم و ژیاردیا از جمله شایع­ترین انگل­های موثر بر ناهنجاری­های روده باریک سگ­ها محسوب می‌شوند. در این مطالعه تعداد 200 نمونه مدفوعی از سگ­های گله و نگهبان به­صورت کاملاً تصادفی از مناطق مختلف روستایی اطراف شهر مهاباد جمع­آوری و پس از تثبیت در محلول­های فرمالینه و پلی­وینیل الکل به آزمایشگاه ارجاع داده شد. نمونه­ها با استفاده از روش­های تغلیظی و رنگ­آمیزی به روش­های تری­کروم گوموری و زیل­نیلسن اصلاح­یافته مورد بازرسی میکروسکوپی با بزرگ­نمایی 1000 برابر از لحاظ وجود تروفوزوئیت، کیست و اووسیست تک­یاخته­ها قرار گرفتند. در مجموع تعداد 9 قلاده (5/4 درصد) و 31 قلاده (5/15 درصد) از سگ­های مورد مطالعه به‌ترتیب به تک­یاخته­های جنس کریپتوسپوریدیوم و ژیاردیا آلوده بودند. بالاترین میزان شیوع کریپتوسپوریدیوم در گروه سنی پایین­تر از 6 ماه (34/10 درصد) و کمترین میزان شیوع در گروه سنی بالای 4 سال (54/1 درصد) مشاهده گردید. همچنین بیشترین میزان آلودگی به ژیاردیا در گروه سنی 6 تا 12 ماه (25 درصد) و کمترین میزان در گروه سنی 1 تا 4 سال (62/8 درصد) یافت شد. میزان شیوع عفونت با کریپتوسپوریدیوم و ژیاردیا در سگ­های نر به­ترتیب 67/4 درصد و 95/14 درصد و در سگ­های ماده به­ترتیب 30/4 درصد و 13/16 درصد ثبت گردید. میزان شیوع هر دو تک­یاخته در سگ­های نر و ماده و همچنین در گروه­های مختلف سنی از لحاظ آماری معنی­دار نبود. همچنین علی­رغم این­که میزان آلودگی با هر دو تک­یاخته کریپتوسپوریدیوم و ژیاردیا در سگ­های با وضع بهداشتی نامناسب بیشتر از گروه واجد وضع مناسب بهداشتی بود ولی این اختلاف از لحاظ آماری معنی­دار نبود.

کلیدواژه‌ها


عنوان مقاله [English]

Prevalence of Cryptosporidium and Giardia in rural herd and guard dogs of Mahabad region

نویسندگان [English]

  • Ali Hayatrouhi 1
  • Mohammad Sadaghian 2
1 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 Assistant Professor, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran.
چکیده [English]

The genera Cryptosporidium and Giardia are among the most common protozoal parasites causing small intestinal abnormalities of dogs.  In this study, 200 fecal samples of herd and guard dogs were collected randomly from different rural areas around Mahabad city and referred to the laboratory after stabilization with formalin and polyvinyl alcohol solutions. Samples were microscopically studied using concentration method followed by the Trichrome-Gumurian and modified Ziehl-Neelsen staining techniques. In general, 9 (4.5%) and 31 (15.5%) of the studied dogs were infected with Cryptosporidium and Giardia respectively. The highest incidence of cryptosporidium was observed in dogs less than 6 months (10.43%) and the lowest incidence was in the age group more than 4 years (1.54%). The highest levels of Giardia infection were found in the 6-12 months age group (25%) and the lowest in the 1-4 years age group (8.62%). The prevalence of infection with cryptosporidium and giardia in male dogs was 4.67% and 14.95%, respectively, and 4.30% and 16.13% in female dogs, respectively. The prevalence of both protozoa in male and female dogs as well as in different age groups was not statistically significant. There were no significant relation between age and gender with prevalence of both parasites. However, the level of infection with both Cryptosporidium and Giardia protozoa in dogs with inappropriate sanitation was higher than those with proper sanitary status, but this difference was not statistically significant.

کلیدواژه‌ها [English]

  • Cryptosporidium
  • Giardia
  • Dog
  • Mahabad
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