1دانش آموخته دانشکده دامپزشکی، دانشگاه فردوسی مشهد، ایران
2گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران
3گروه بهداشت مواد غذایی و آبزیان دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران.
4گروه علوم درمانگاهی - بهداشت و پیشگیری بیماریهای دامی دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران.
چکیده
با توجه به تجمع فلز سرب در بافتهای خوراکی ماهی، امنیت و سلامت غذای انسان نیز تحت تاثیر قرار میگیرد. مطالعه حاضر به منظور مقایسه تجربی اثرات اسیدآسکوربیک و تیامیندر جلوگیری از ضایعات بافتی ناشی از سرب در برخی بافتهای ماهی کپور انجام شد. ماهیان بهصورت تصادفی به 4 گروه 30تایی تقسیم شدند. گروه 1 به عنوان شاهد در نظر گرفته شد. گروه 2 استات سرب، گروه 3 و 4 علاوه بر سرب به ترتیب تیامین و اسید آسکوربیک نیز دریافت کردند. پس از کالبدگشایی، نمونههای بافتی از مغز، کبد و کلیه تهیه و برشهای آماده شده، بهروش هماتوکسیلین- ائوزین رنگآمیزی شد. در مقایسه آماری ضایعات بافت مغز شامل پرخونی، ادم و تغییرات ایسکمیک در گروه 4 به صورت معنیداری کمتر از گروههای 2 و 3 بود. همچنین در کبد، پرخونی، هایپرپلازی کانونهای ملانوماکروفاژ، تغییرات دژنراتیو و گنجیدگیهای اسیدفست داخل هستهای هپاتوسیتها مشاهده گردید. شدت پرخونی در بافت کبد و تغییرات دژنراتیو هپاتوسیتها در گروه 3 بهصورت معنیداری کمتر از گروه 4بود. هیچیک از شاخصهای پاتولوژیک کلیه شامل پرخونی، خونریزی، گنجیدگیهای اسیدفست داخل هستهای، تورم و نکروز سلولهای پوششی توبولها و هایپرپلازی کانونهای ملانوماکروفاژ، بین گروههای 3 و 4 تفاوت معنیداری نشان ندادند، اما در مقایسه برخی ضایعات این دو گروه با گروه 2، اختلاف معنیدار مشاهده گردید.بر پایه نتایج ذکر شده میتوان نتیجهگیری نمود که اسید آسکوربیک و تیامین میتوانند در پشیگیری و درمان مسمومیت با سرب به عنوان یک گزینه مناسب در ماهیان مطرح باشند.
Experimental comparison of the effectsof ascorbic acid and thiamine in prevention of lead induced tissue damages in selected tissues of common carp (Cyprinus carpio)
نویسندگان [English]
Fatemeh Pourali1؛ Zahra Moosavi2؛ Davar Shahsavani3؛ Mohammad Azizzadeh4
1D.V.M. Graduate, Faculty of Veterinary Medicine, Ferdowsi University of Mashad, Mashad, Iran.
2Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashad, Mashad, Iran.
3Professor, Department of Fish Medicine, Faculty of Veterinary Medicine. Ferdowsi University of Mashad, Mashad, Iran.
4Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashad, Mashad, Iran.
چکیده [English]
Due to accumulation of lead in edible tissues of fish, the safety and health of human food are also affected. This study was conducted to compare the effects of ascorbic acid and thiamine in prevention of lead induced tissue damages in selected tissues of common carp (Cyprinus carpio). Fish were divided randomly into 4 groups of 30 fish each. Group 1 was considered as control group. Group 2 received lead acetate while groups 3 and 4 received thiamine and ascorbic acid respectively in addition to lead. After necropsy, tissue specimens were collected from the brain, kidney and liver and the processed slides were stained with hematoxylin and eosin. Brain lesions in group 4 consisting of hyperemia, edema and ischemic cell changes were significantly lower in comparison to groups 2 and 3. In liver, hyperemia, hyperplasia of melano-macrophage centers, hepatocellular degeneration and intranuclear acid-fast inclusion bodies were observed. The severity of hyperemia and degeneration in group 3 was significantly lower in comparison to group 4. In the statistical comparison, none of the renal pathological indices including hyperemia, hemorrhage, intranuclear acid-fast inclusion bodies, necrotic changes, swelling of epithelial cells and hyperplasia of melano-macrophage centersrevealed significant differences between groups 3 and 4 but compared to group 2, some lesions in these groups showed a significant difference. Based on the results, it can be concluded that ascorbic acid and thiamin might have some protective and therapeutic effects on lead poisoning in fish.
کلیدواژهها [English]
lead, Thiamine, Ascorbic acid, Histopathology, Common carp
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