تاثیر مکمل پره‌بیوتیک سل‌ماناکس بر بافت آبشش و کبد ماهی قزل‌آلای رنگین‌کمان در دوره پرورشی و مواجهه تجربی با بیماری یرسینیوزیس

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه بهداشت، بهداشت و بیماری های آبزیان، دانشکده علوم تخصصی دامپزشکی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران

2 دانشیار گروه پاتولوژی، دانشکده علوم تخصصی دامپزشکی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران

3 استاد گروه فارماکولوژی و سم شناسی، دانشکده داروسازی، دانشگاه ارومیه، ارومیه، ایران استاد مرکز تحقیقات سلامت مواد غذایی و آشامیدنی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران

4 دانشیار گروه باکتری شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

5 استادیار گروه بهداشت، بهداشت و بیماری های آبزیان، دانشکده علوم تخصصی دامپزشکی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران

چکیده

امروزه استفاده از ترکیبات پره­بیوتیکی در جهان گسترش فراوانی یافته ­است. هدف از این مطالعه بررسی اثرات تغذیه­ای غلظت­های مختلف محصول تجاری پره­بیوتیک سل­ماناکس (حاوی ترکیبات فعال دیواره مخمر ساکارومایسیس سرویزیه به همراه مانان الیگوساکارید) بر بافت­های آبشش و کبد ماهیان تغذیه­شده با پره­بیوتیک مذکور در طول دوره پرورش و در مواجهه تجربی با بیماری یرسینیوزیس بود. ماهیان قزل­آلای رنگین­کمان با میانگین وزنی 45/1±08/19 گرم به­مدت 60 روز با جیره حاوی غلظت­های مختلفپره­بیوتیک سل­ماناکس (0، 1/0، 5/0 و 1 درصد) تغذیه شدند. در روز 60 مطالعه،بیماری یرسینیوزیس توسط تزریق داخل صفاقی سوسپانسیون باکتری،در همه گروه­های آزمایشی به صورت تجربی ایجاد گردید. نمونه­های بافت­شناسی در روزهای صفر، 30 و 60 مطالعه و همچنین پس از آزمون مواجهه تجربی تهیه شد.­ نتایج نشان­دهنده تاثیر غلظت­های مختلف پره­­بیوتیک سل­ماناکس بر بافت‌های کبد و آبشش در روز­های 30 و 60 مطالعه بود. بهترین تاثیر بافتی در دوره پرورشی مربوط به تیمار­های تغذیه­شده با غلظت‌های 1/0 و سپس 5/0 درصد بود و نیز در زمان مواجهه تجربی با بیماری یرسینیوزیس کمترین جراحات بافتی کبد و آبشش‌ها مربوط به تیمار تغذیه­شده با غلظت 1/0 درصد پره­بیوتیک سل­ماناکس بود که از لحاظ آماری دارای تفاوت معنی­داری با سایر گروه­ها بود (05/0>p). با توجه به نتایج این بررسی مشخص شد که افزودن پره­بیوتیک سل­ماناکس به جیره ماهی قزل­آلای رنگین­کمان با غلظت 1/0 درصد، سبب بهبود پارامترهای بافتی در طول دوره پرورشی و کاهش ضایعات بافتی در زمان مواجهه با بیماری یرسینیوزیس می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of dietary supplement of celmanax® prebiotic on gill and liver histology in Rainbow trout during the growing period and experimental challenge with yersiniosis

نویسندگان [English]

  • amin Khodadadi 1
  • Adel Haghighi 2
  • Hasan Malekinejad 3
  • Amir Tukmechi 4
  • Mohamad Afsharnasab 5
1 Department of Health, Aquatic Animal Health and Disease, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, IRAN
2 Associate Professor, Department of Pathology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Professor, Department of pharmacology and toxicology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran Professor, Food and beverages safety Research center, Urmia University of Medical Sciences, Urmia, Iran
4 Associate Professor, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5 Assistant Professor, Department of Health, Aquatic Animal Health and Disease, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, IRAN
چکیده [English]

Currently, global use of  prebiotic compounds has increased considerably. This study aims to analyse the effect of complementing the rations of rainbow trout with different concentrations of Celmanax® prebiotic, which contains Saccharomyces cerevisia associated compounds with Mannan-oligosaccharide on gill and liver histology during the growing period and experimental challenge with yersiniosis. Four concentration levels of prebiotic (0, 0.1, 0.5 and 1%) were mixed into fish feed pellets. The fish (with mean body weight of 19.08±1.45 gr) were fed a supplemented commercial diet for 60-days.On day 60 of the study, experimental yersiniosis was induced in all treatment groups by intraperitoneal injection of bacterial suspension. Tissue samples were taken on days 0, 30 and 60 and also after induction of experimental yersiniosis. The results showed that complementing rainbow trout rations with different concentrations of Celmanax®  improved the gill and liver injuries on days 30 and 60 of the study. The best tissue effects in the growing period were observed in treatment groups which received  0.1% and  0.5% of the prebiotic respectively, and after experimental challenge with yersiniosis, the lowest lesions of liver and gill pathology were seen in the treatment group fed with 0.1% prebiotic which was significantly different (p<0.05) from all other treatments. According to the results of this study, adding Celmanax® to rainbow trout diet at 0.1% concentration improves tissue parameters during breeding period and decreases tissue lesions when faced with yersiniosis.
Conflict of interest: None declared.

کلیدواژه‌ها [English]

  • prebiotics
  • Yersiniosis
  • Rainbow trout
  • Gill
  • liver
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