بررسی اثر هم‌زمان‌سازی فحلی و استفاده از GnRH بر برخی شاخص‌های سرمی و میزان باروری گوسفندان نژاد ماکویی در فصل تولیدمثل

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشگاه تبریز

2 استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

3 استاد گروه علوم دامی دانشکده کشاورزی دانشگاه تبریز

4 هیئت علمی دانشگاه تبریز

5 دانشجوی سابق کارشناسی ارشد گروه علوم دامی دانشگاه تبریز

6 رزیدنت بیماری‌های داخلی دام‌های بزرگ، دانشکده دامپزشکی، دانشگاه تهران.

چکیده

یکی از مهم‌ترین مشکلات دامداری­ها پایین بودن نرخ باروری است. بنابراین، می­توان با استفاده از هورمون­های صناعی فرآیند تولیدمثل را در دام­ها کنترل و نرخ باروری آن­ها را با استفاده از هم­زمان­سازی فحلی افزایش داد. هدف از انجام این مطالعه بررسی اثر هورمون GnRH(هورمون آزادکننده گنادوتروپین) در روزهای 10، 11 و 12 بعد از تلقیح مصنوعی بر باروری میش بود. برای این منظور 226 رأس گوسفند ماکوئی با استفاده از سیدر همزمان­سازی فحلی شدند. پس از درآوردن سیدر، تمامی میش­ها  PMSG(گنادوتروپین سرم مادیان آبستن) را به مقدار 400 واحد بین­المللی دریافت کردند. دو روز بعد، میش­های فحل­شده با اسپرم تازه، تلقیح مصنوعی شده و به سه گروه تقسیم شدند. گروه شاهدهیچ هورمونی دریافت نکرد. گروه دوم به زیرگروه­های D، E و Fتقسیم شدند که به ترتیب در روزهای 10، 11 و 12 بعد از تلقیح مقدار 20 میکروگرم GnRH(هورمون آزادکننده گنادوتروپین‌) به­روش عضلانی دریافت کردند و گروه سوم (گروه G) نیز در روز 12 بعد از تلقیح مقدار 40 میکروگرم GnRH دریافت کرد. 5 میلی­لیتر خون دو روز بعد از تزریق به­وسیله لوله ونوجکت گرفته شد. غلظت گلوکز، فسفر، اوره، پروتئین تام و پروژسترون سرم خون اندازه­گیری گردید. برای تجزیه متغیرهای وابسته و متغیرهای مستقل به ترتیب از رویه GLM(General Linear Model) و FREQ
(
frequencies) نرم­افزار SASاستفاده شد. نتایج نشان داد که اثر GnRH بر میزان پروژسترون کاملاً معنی­دار بود (01/0>p). همچنین بیشترین نرخ باروری متعلق به گروه سوم بوده و تفاوتی معنی­دار در نرخ باروری بین گروه مذکور با سایر گروه­ها مشاهده شد (01/0>p). به­طور کلی می­توان نتیجه گرفت که تزریق 5 میلی‌لیتر GnRH در روز 12 بعد از تلقیح، نرخ باروری میش را بهبود می‌بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of estrus synchronization and GnRH on some serumic indices and pregnancy rate of Makui sheep during the breeding season

نویسندگان [English]

  • rasoul babazadeh aghdam 1
  • Gh Moghaddam 2
  • hossein daghighkia 3
  • Seyyed Abbas Rafat 4
  • aytak bakhshayesh khiabani 5
  • S Moghaddam 6
1 Tabriz university
2 Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
3 Professor, department of animal Science, University of Tabriz, Tabriz, Iran
4 Professor, department of animal Science, University of Tabriz, Tabriz, Iran
5 Former MSc student of Animal Science, University of Tabriz, Tabriz, Iran
6 Resident of Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
چکیده [English]

Low pregnancy rate is an important problem in livestock. Therefore it is possible to control the process of reproduction in animals by using synthetic hormones and increase the pregnancy rate through estrus synchronization. The aim of this study was to investigate the effect of GnRH on days 10, 11, and 12 after artificial insemination on pregnancy rate of Makui sheep breed. For this purpose, estrus was synchronized in 226 ewes using CIDR. After removing CIDR, all ewes received 400 IU PMSG. After two days, ewes in estrus were artificially inseminated with fresh sperms and divided into three groups. The control group did not receive any hormone. The second group was divided into subgroups D, E and Fwhich received 20 µg GnRH on days 10, 11, and 12 after artificial insemination respectively. Finally, the third group or group G received 40 µg GnRH on day 12 after artificial insemination. Blood samples (5 ml) were collected two days after GnRH injection by using vacuum tubes. The serumic concentrations of glucose, urea, total protein, phosphorus and progesterone were measured. To analyze the dependent and discrete variables, GLM and FREQ procedure of SAS software were used respectively. The results showed that the effect of GnRH on plasma concentration of progesterone was significant (p<0.01). Maximum pregnancy rate was achieved in the third group with a significant difference (p<0.01) observed between this group and the other groups regarding pregnancy rate. It can be concluded that injection of 40 µg of GnRH hormone on day 12 after insemination improves pregnancy rate in ewes.

کلیدواژه‌ها [English]

  • estrus synchronization
  • GnRH
  • Blood serum indices
  • pregnancy
  • Sheep
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