بررسی عیار سرمی آنتی‌بادی ضد مایکوباکتریوم‌ اویوم زیرگونه پاراتوبرکلوزیس به‌روش الایزا در گاوهای شیری هلشتاین

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

2 استادیار گروه میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

3 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

چکیده

بیماری یون از جمله عفونت­های مزمن باکتریایی در نشخوارکنندگان میباشد که با توجه به اسهال شدید و طولانی­مدتی که ایجاد می‌کند، باعث تحلیل رفتن بدن دام می­شود. هدف از این مطالعه، بررسی عیار سرمی آنتی­بادی ضد مایکوباکتریوم اویوم زیرگونهپاراتوبرکلوزیس (عامل بیماری یون) به روش الایزا و برآورد میزان آلودگی به عامل مذکور در گاوهای شیری مجتمع دامپروری کشت­و­صنعت مغان بود. در این مطالعه از تعداد 738 رأس گاو شیری بالغ در مجتمع کشت­و­صنعت و دامپروری مغان که به­طور تصادفی انتخاب شدند، خون­گیری شده و نمونه­های سرمی در آزمایشگاه به­روش الایزا بررسی شدند. مطالعه حاضر نشان داد از تعداد 738 رأس گاو مورد آزمایش، 58 رأس دارای عیار سرمی علیه مایکوباکتریوم اویوم زیرگونهپاراتوبرکلوزیس با درصد S/P بیشتر از 60 درصد بودند (میزان شیوع 86/7 درصد) که 25 رأس مثبت (با درصد S/P بیشتر از 70 درصد) و 33 رأس مشکوک (با درصد S/P بیشتر از 60 تا 70 درصد) بودند. به دلیل این که چهره بیماری یون اغلب تحت بالینی بوده و دام در ­ظاهر سالم به­نظر می­رسد، به سبب دفع میکروب در مدفوع و ترشحات بدن خود بقیه گله را نیز آلوده می­کند. بنابراین، انجام آزمون­های غربالگری دوره­ای در گله و همچنین تست دام­های تازه وارد در کنار سایر اقدامات پیشگیرانه اکیداً توصیه می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle

نویسندگان [English]

  • yavar heydarzadeh Asiabi 1
  • Behboud Jafari 2
  • Ali hassanpour 3
1 MSc. Student of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran.
2 Assistant Professor, Department of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran.
3 Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
چکیده [English]

Paratuberculosis (Johne’s disease) is achronic bacterial infection of ruminants causing body wasting due to severe and protracted diarrhea. The aim of the present study was to investigate the rate of infection to Johne’s disease in cattle of Moghan agro-business and animal husbandry complex by detecting antibody titers against Mycobacterium avium subspecies paratuberculosis (MAP) using the absorptive ELISA method. For this purpose, blood samples were collected from 738 randomly selected milking cattle of Moghan agro-business and animal husbandry complex and their sera were assessed by ELISA method. The results indicated that 58 cattle had antibody titers against MAP with S/P percentage over 60% (infection rate of 7.86%) of which 25 cattle were positive (with S/P percentage over 70%) and 33 were suspicious (with S/P percentage between 60-70%). Because Johne’s disease is manifested as a subclinical disease and infected cattle seem to be apparently healthy, bacteria are shed through faeces and body secretions causing infection of the rest of the herd. Thus performing periodic herd screening tests and testing recently introduced animals alongside other preventive measures is strictly advised.

کلیدواژه‌ها [English]

  • Mycobacterium avium subspecies paratuberculosis
  • Antibody
  • Elisa
  • dairy cattle
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