بررسی اثر عصاره گیاه کرفس (Apium graveolens) بر میزان کلسیم و اگزالات سرم در آسیب کلیوی ناشی از اتیلن ‌گلیکول در موش‌های صحرایی نر بالغ نژاد ویستار

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 دانشیار پاتولوژی، گروه پاتولوژی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

کرفس (Apium graveolens) گیاهی دوساله و از خانواده Apiaceae است. بخش­های مختلف کرفس در اختلالات کبدی و طحال، اختلالات مغزی، درد و اختلالات خواب استفاده می­شود. مطالعه حاضر به منظور بررسی اثر عصاره اتانولی کرفس بر آسیب کلیه ناشی از اتیلن­ گلیکول در موش­های صحرایی نر نژاد ویستار انجام گرفت.القاء سنگ­کلیه با استفاده از تیمار 75/0 درصد اتیلن­ گلیکول به مدت 28 روز انجام شد. موش­ها به 8 گروه 6تایی تقسیم شدند که به ترتیب شامل کنترل سالم، کنترل سنگ کلیوی (75/0 درصد اتیلن­ گلیکول در آب آشامیدنی)، حیوانات سالم تیمار با عصاره کرفس در دوزهای 50، 100 و 200 میلی­گرم بر کیلوگرم و موش­های سنگ کلیویتیمار با عصاره کرفس در دوزهای 50، 100 و 200 میلی­گرم در کیلوگرم وزن بدن به صورت گاواژ بودند. در پایان آزمایش (28 روز)، نمونه خون از قلب موش­ها جمع­آوری شد و میزان کلسیم و اگزالات در سرم ارزیابی گردید. واکاوی آماری از طریق آزمون تحلیل واریانس یک­طرفه و آزمون تعقیبی توکی انجام شد و 05/0p< معنی­دار در نظر گرفته شد.افزایش معنی­دار میزان اگزالات و کاهش معنی­دار کلسیم سرم در حیوانات گروه کنترل سنگ کلیوی در مقایسه با گروه کنترل سالم مشاهده شد. تیمار عصاره کرفس با غلظت 200 میلی­گرم بر کیلوگرم وزن بدن باعث کاهش معنی­دار اگزالات و افزایش معنی­دار کلسیم سرم در حیوانات دچار سنگ کلیوی شد.نتایج مطالعه حاضر نشان داد که عصاره کرفس می­تواند باعث بهبود سنگ ­کلیه ناشی از اتیلن ­گلیکول شود.
 

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Apium graveolens extract on serum calcium and oxalate in ethylene glycol- induced kidney injury in male Wistar rats

نویسندگان [English]

  • Parisa Mehrparvar 1
  • Akram Eidi 1
  • Pejman Mortazavi 2
  • Shahrbanoo Oryan 1
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Pathology, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Celery (Apium graveolens) is a biennial plant, belonging to family Apiaceae. Various parts of celeryare used in hepatic and spleen disorders, brain disorders, body pain, and sleep disturbances. The present study was planned to investigate the effect of celery ethanolic extract on ethylene glycol induced kidney injury in male Wistar rats. Urolithiasis was induced in hyperoxaluric rat model by giving 0.75% ethylene glycol for 28 days. Animals were divided into 8 groups (n=6) including normal control, urolithiatic control (0.75% ethylene glycol in their drinking water), normal rats receiving celery extract at doses of 50, 100 and 200 mg/kg and urolithiatic rats receiving celery extract at doses of 50, 100 and 200 mg/kg intragastrically. At the end of the experiment (28 days), blood samples were collected by cardiac puncture and serum calcium and oxalate was analyzed. Statistical analysis was performed by one-way analysis of variance followed by Tukey post hoc test (p<0.05). Significant elevation of serum oxalate and reduction of serum calcium was observed in urolithiatic control rats compared with the normal control rats. Administration of celery extract at a dose of 200 mg/kg produced significant elevation in serum calcium and reduction in serum oxalate in urolithiatic rats. Thus, celery extract could ameliorate urolithiasis induced by ethylene glycol.

کلیدواژه‌ها [English]

  • Celery
  • Urolithiasis
  • Ethylene glycol
  • Rat
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