اثر تزریق درون‌تخم‌مرغی نسبت‌های مختلف دی‌ال-متیونین به ال-لایزین بر هیستومورفولوژی روده کوچک و اندام‌های سیستم ایمنی در جوجه‌های گوشتی یک‌روزه

نوع مقاله: علمی پژوهشی

نویسندگان

1 عضو هیات علمی گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

2 دانش آموخته کارشناسی ارشد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

3 استادگروه بافت شناسی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

4 دانشیارگروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران

چکیده

توسعه زودهنگام روده کوچک می‌تواند موجب افزایش نرخ رشد در جوجه‌های تازه هچ‌شده شود. بنابراین، هدف از این مطالعه بررسی اثر تزریق درون­تخم‌مرغی نسبت‌های مختلف دی‌ال-متیونین به ال-لایزین بر هیستومورفولوژی روده کوچک و اندام‌های سیستم ایمنی در جوجه­های گوشتی یک روزه راس 308 بود. در این مطالعه از 210 تخم‌مرغ بارور مادر گوشتی راس 308 در قالب طرح کاملاً تصادفی با 7 گروه آزمایشی شامل 30 تخم­مرغ انفرادی استفاده شد. گروه­های آزمایشی شامل شاهد (بدون تزریق)، شم-شاهد (1 میلی‌لیتر آب مقطر)، نسبت‌های 5/40، 5/45، 5/50، 5/55 و 5/60 درصد دی‌ال-متیونین به ال-لایزین بودند که در روز 14 دوره انکوباسیون از قسمت پهن تخم­مرغ به مایع آمنیوتیک تزریق شدند. در روز 22 مطالعه، ابتدا همه جوجه‌ها وزن‌کشی و کشتار شدند تا شاخص­های مربوط به بافت روده و اندام‌های سیستم ایمنی اندازه­گیری شوند. بر اساس نتایج آزمایش حاضر، اثر تزریق درون تخممرغی نسبت­های مختلف دی‌ال-متیونین به ال-لایزین بر وزن و وزن نسبی ایلیوم، طول ایلیوم، وزن و وزن نسبی روده کوچک، طول و طول نسبی روده کوچک معنی‌دار و افزایش‌دهنده بود (01/0p<). همچنین اثر معنی‌دار (05/0p<) تیمارها بر قطر کریپت (به جز دوازدهه)، عمق کریپت، طول پرز و نسبت طول پرز به عمق کریپت دوازدهه، ژژنوم و ایلیوم مشاهده شد. از سوی دیگر، اندام‌های سیستم ایمنی تحت تأثیر تیمارها قرار نگرفتند. نتایج کلی این مطالعه نشان داد تزریق درون­تخم‌مرغی از نسبت 5/40 تا 5/50 درصد دی‌ال-متیونین به ال-لایزین، بهبود رشد و هیستومورفولوژی روده کوچک جوجه‌های گوشتی را ایجاد می­کند.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of in ovo injection of different DL-methionine to L-lysine ratios on small intestinal histomorphology and immune system organs in day-old broiler chicks

نویسندگان [English]

  • Marziyeh Ebrahimi 1
  • Roya Ghochkhani 2
  • Masoud Adibmoradi 3
  • Zolfaghar Rajabi 4
1 Department of Animal Science, Faculty of Agriculture, University of Tabriz, 29 Bahman Boulevard, Imam Khomeini street, P.O.Box# 5166616471, Tabriz, East Azerbaijan, Iran. Tel/Fax: +984113356004.
2 MSc. Graduated at Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Professor at Department of Histology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 Associate professor at Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده [English]

Early development of small intestine can cause higher growth rate in hatchlings. Therefore, the aim of this study was to evaluate the effect of in ovo injection of different ratios ofDL-methionine to L-lysine on small intestinal histomorphology and immune system organs of day-old Ross 308 broiler chicks. In this experiment, 210 fertile Ross 308 broiler breeder eggs were randomly assigned into 7 experimental groups including 30 individual eggs per group. Treatment groups included: sham control (non-injected), control (1ml distilled water), 40.5, 45.5, 50.5, 55.5, and 60.5% DL-methionine to L-lysine ratios which were injected into the amnion at 14 d of incubation. On day 22, hatched chicks were weighed and slaughtered in order to evaluate the characteristics of small intestine and immune system organs. Based on the results of the present experiment, the effects of in ovo injection with different DL-methionine to L-lysine ratios on weight and relative weight of ileum, ileum length, weight and relative weight of small intestine, length and relative length of small intestine were significant (p<0.01) and elevatory. Also, significant effect (p<0.05) of treatments was observed on crypt diameter (except for duodenum), crypt depth, villus height, and villus height to crypt depth ratio in duodenum, jejunum, and ileum. On the other hand, immune system organs were not affected by the treatments. The overall results of the present study showed that in ovo injection from 40.5 to 50.5% of DL-methionine to L-lysine ratio improved small intestinal growth and histomorphology in broiler chicks.

کلیدواژه‌ها [English]

  • Small intestine
  • In ovo injection
  • Broiler chick
  • DL-methionine to L-lysine ratio
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