بررسی عوامل باکتریایی ورم پستان تحت‌بالینی در گاوهای شیری مجتمع کشت‌وصنعت و دامداری نگین سبز ماکو

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

2 استادیار گروه میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

3 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

چکیده

بروز عفونت پستانی در گاو و کاهش مقاومت این حیوان نسبت به عوامل بیماریزا منجر به بروز فرم حاد و بالینی ورم پستان شده و این عارضه به عنوان یکی از مسائل مهم در پرورش گاو به شمار می رود هدف از انجام این تحقیق شناسایی عوامل ایجاد کننده ی ورم پستان تحت بالینی می­باشد تا به عنون اولین قدم برای پیشگیری از خسارات بهداشتی و اقتصادی باشد. در گاوداری روزانه به طور مرتب و در نوبت دوشش صبح. پس از نمونه گیری هم حجم شیر به آن محلول شیر آزما به عنوان معرف اضافه نموده و مخلوط شیر و معرف به مدت چند دقیقه به صورت دورانی همزده خواهد شد. لخته شدن مخلوط شیر و معرف موید وجود ورم پستان تحت بالینی می­باشد. نمونه­های جمع آوری شده در هر نوبت دوشش، در کنار یخ به آزمایشگاه میکروبیولوژی ارسال شده و کشت میکروبی و آنتی بیوگرام صورت گرفت.بیشترین باکتری­های جدا­شده شامل کورینه باکتری (54/12 درصد)، استافیلوکوکوس اورئوس (92/7 درصد)، استرپتوکوکوس آگالاکتیه (62/4 درصد)، استافیلوکوکوس ساپروفیتیکوس (62/4 درصد)، استافیلوکوکوس اپیدرمیس (3/3 درصد)، باسیلوس (64/2 درصد)، استرپتومایسس (98/1 درصد) بودند.ارزیابی حساسیت آنتی بیوتیکی، بیشترین حساسیت را به جنتامایسین و بیشترین مقاومت را به پنی­سیلین نشان داد. نتایج مطالعه نشان­دهنده مقاومت جدایه­های باکتریایی نسبت به آنتی­بیوتیک­های پرمصرف مانند پنی­سیلین، استرپتومایسین و تا حدی تتراسایکلین بود، ولی حساسیت به جنتامایسین که کمتر مورد استفاده قرار گرفته است، بالا بود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of bacterial causes of subclinical mastitis in dairy cattle of Negine sabze Makoo agro-industrial and animal husbandry complex

نویسندگان [English]

  • mehdi shokohi 1
  • changiz ahmadizadeh 2
  • amir ali kaveh 3
1 Masters Student of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran.
2 Assistant Professor, Department of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran.
3 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
چکیده [English]

Acute clinical mastitis resulting from udder infections and reduced resistance to pathogenic organisms is an important issue in cattle production. The aim of this study was to identify the causes of subclinical mastitis as a first step in prevention of hygienic and economic losses. Daily milk samples were collected every morning after milking and mixed with equal volume of mastitis test reagent and the mixture was rotated in a figure of 8 fashion for a few minutes. Presence of subclinical mastitis was confirmed by clotting of this mixture. The collected samples from each milking were sent to the microbiology laboratory alongside ice for bacterial culture and antibiotic susceptibility tests. Results indicated that the highest number of isolated bacteria consisted of Corynebacteria (12.54%), Staphylococcus aureus (7.92%), Streptococcus agalactiae (4.62%), Staphylococcus saprophyticus (4.62%), Staphylococcus epidermis (3.3%), Bacillus (2.64%) and Streptomyces (1.98%). Antibiotic susceptibility test revealed the greatest susceptibility to gentamicin and resistance to penicillin indicating the presence of resistance in isolated bacteria against commonly used antibiotics like penicillin, streptomycin and to some extent tetracycline but susceptibility towards the lesser used gentamicin was high.

کلیدواژه‌ها [English]

  • subclinical mastitis
  • dairy cattle
  • Negine sabze Makoo agro-industrial and animal husbandry complex
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