جداسازی سالمونلا از مزارع و خوراک مرغ مادر گوشتی ایران

نوع مقاله: علمی پژوهشی

نویسندگان

1 استاد گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

2 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

3 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

4 دانشجوی دکترای تخصصی، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.

چکیده

آلودگی طیور با باکتری­های جنس سالمونلا یکی از مسائل مهم در زمینه سلامت انسان و طیور محسوب می­شود و ماکیان نقش مهمی در انتشار و شیوع سالمونلوز در انسان دارند. هدف این مطالعه جداسازی سالمونلا از مزارع و خوراک مرغ مادر گوشتی ایران می‌باشد. بدین منظور از 62 گله­ مرغ مادر گوشتی و خوراک آن­ها در 21 استان کشور در محدوده زمانی یک­ساله نمونه­برداری صورت گرفت و با استفاده از روش کشت مرسوم شامل مراحل پیش­غنی­سازی، غنی­سازی و کشت در محیط­های اختصاصی و تفریقی، تعداد 18 جدایه سالمونلا تشخیص داده شد. جهت تأیید هویت سالمونلای تشخیص داده شده از روش واکنش زنجیره­ای پلیمراز چندگانه استفاده شد و 12 جدایه سالمونلا تأیید گردید که 7 جدایه (33/58 درصد) مربوط به سالمونلا انتریتیدیس در استان­های قزوین، مازندران و مرکزی، 3 جدایه (25 درصد) مربوط به سالمونلا اینفانتیس در استان­های کردستان و جنوب خراسان و 2 جدایه (66/16 درصد) مربوط به سالمونلا تیفی موریوم در استان­های فارس و لرستان مشخص گردید. تمامی نمونه‌های خوراک از نظر آلودگی به باکتری‌های جنس سالمونلا منفی بودند. نتایج این مطالعه نشان داد تعدادی از مزارع مرغ مادر ایران به باکتری سالمونلا آلوده می­باشند و سالمونلای غالب در مزارع مرغ مادر گوشتی ایران به ترتیب سالمونلا انتریتیدیس، سالمونلا اینفانتیس و سالمونلا تایفی­موریوم می‌باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

Isolation of Salmonella from Iranian broiler breeder farms and feed

نویسندگان [English]

  • Mansour Mayahi 1
  • Forough Talazade 2
  • Ramezan Ali Jafari 3
  • Vahid Keshavarz Zamanian 4
1 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4 PhD Student, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده [English]

Contamination of poultry by salmonella spp. is an important issue both in the field of public health as well as in the poultry industry and poultry have a significant role in transmission and incidence of human salmonellosis. The aim of the present study was isolation and identification of Salmonella spp. in Iranian broiler breeder farms and their feed. Samples from Sixty two broiler breeder farms and their feed from 21 states of Iran were collected during one year. All samples were cultured in different conventional media, including pre-enrichment, enrichment, selective plating and 18 Salmonella isolates were identified. Salmonella identification was confirmed by multiplex PCR and 12 isolates were confirmed. Out of positive samples, seven samples (58.33%) were Salmonella enteritidis in Ghazvin, Mazandaran and Markazi provinces, three samples (25%) were Salmonella infantis in Kordestan and southern Khorasan provinces, and two samples (16.6%) were Salmonella typhimurium in Fars and Lorestan provinces. All feed samples were negative. The results of this study showed that some breeder farms in Iran are contaminated with Salmonella and the most prominent Salmonella inbroiler breeder farms are Salmonella enteritidis, Salmonella infantis, and Salmonella typhimurium respectively.

کلیدواژه‌ها [English]

  • Salmonella isolation
  • Broiler breeder farms
  • feed
  • Multiplex PCR
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