تاثیر نانو‌ذرات سلنیوم و سلنیت ‌سدیم بر شاخص‌های ایمنی هومورال بلدرچین‌های تحت-استفاده از غذاهای آلوده به آفلاتوکسین B1

نوع مقاله: علمی پژوهشی

نویسندگان

1 استادیار گروه مهندسی علوم دامی، واحد داراب، دانشگاه آزاد اسلامی، داراب، ایران.

2 کارشناسی ارشد میکروب‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران.

3 کارشناسی ارشد ژنتیک، دانشگاه علوم پزشکی تهران، واحد بین الملل، کیش، ایران.

4 کارشناسی ارشد مهندسی پلیمر، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران.

چکیده

سلنیوم یک ماده معدنی کمیاب و ضروری برای سلامت طیور و نیز یکی از بیوکاتالیزورها و اجزای پر‌شمار عملکرد آنزیم‌هاست که جهت بهبود عملکرد سیستم ایمنی، سلامتی و عملکرد تولیدی لازم است. مطالعه حاضر به منظور بررسی قابلیت مهار آفلاتوکسین B1   توسط دو منبع مختلف سلنیومی و مقایسه اثر نانو­سلنیوم و سلنیت ‌سدیم بر ایمنی هومورال بلدرچین­ها انجام گردید. تعداد 240 قطعه جوجه بلدرچین در شش گروه آزمایشی شامل 1- کنترل، بدون آفلاتوکسینB1 و بدون نانو­سلنیوم و سلنیت ­سدیم، 2- با ppm1 آفلاتوکسینB1 و بدون نانو­سلنیوم و سلنیت ­سدیم، 3- ppm1 آفلاتوکسین B1با ppm3/0 نانو­سلنیوم، 4- ppm 1 آفلاتوکسین  B1 با ppm3/0 سلنیت ­سدیم، 5- ppm 1 آفلاتوکسین B1با ppm6/0 نانو­سلنیوم، 6- ppm  1 آفلاتوکسین B1 با ppm6/0 سلنیت ­سدیم، و در قالب طرح بلوک­های کاملاً تصادفی در 4 تکرار و هر تکرار شامل 10 قطعه جوجه اختصاص یافتند. به منظور بررسی پاسخ ایمنی هومورال 2/0 میلی­لیتر سوسپانسیون گلبول قرمز گوسفند در 35 روزگی به عضله سینه بلدرچین تزریق و یک هفته بعد از تزریق خون­گیری انجام شد. واکسن نیوکاسل در 28 روزگی تزریق و دو هفته بعد تیتر آنتی­بادی تعیین شد. بالاترین میزان تیتر آنتی­بادی علیه سوسپانسیون گلبول قرمز گوسفند (SRBC) مربوط به گروه دریافت کننده ppm­6/0نانوسلنیوم بود (01/0p<). نتایج نشان داد ‌که نانو­سلنیوم در مقایسه با سلنیت ­سدیم منجر به بهبود سیستم ایمنی هومورال و پارامتر­های بیوشیمیایی سرم می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of nano-selenium particles and sodium selenite on humoral immunity indices of quails using foods contaminated with aflatoxin B1

نویسندگان [English]

  • Ebrahim Talebi 1
  • Afsaneh Abedi 2
  • Ehsan Rahimi 3
  • Maryam Khosravinezhad 4
1 Assistant Professor, Darab Branch, Islamic Azad University, Darab, Iran.
2 M.Sc., Graduate in Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
3 M.Sc., Graduate in Genetic, Tehran University of Medical Science, International Campus, Kish, Iran.
4 M.Sc., Graduate in Polymer Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
چکیده [English]

This experiment was conducted to evaluate the ability of inhibition of aflatoxin B1 by various sources of selenium and to compare the effect of nano selenium and sodium selenite on humoral immunity of quails. The experiment was performed in a completely randomized design (CRD) using six treatments and four replicates of ten quail chicks per replicate. Two hundred forty quails were divided in six groups vis. control: without aflatoxin B1 and without selenium. Group2: 1ppm aflatoxin B1 and without selenium. Group3: 1 ppm aflatoxin B1 and 0.3 ppm nano selenium. Group4: 1 ppm aflatoxin B1 and 0.3 ppm sodium selenite. Group5: 1 ppm aflatoxin B1 and 0.6 ppm nano selenium. Group6: 1ppm aflatoxin B1 and 0.6 ppm sodium selenite. To evaluate the humoral immunity response 0.2­ml of sheep red blood cell (SRBC) solution was injected into breast muscle of quails at day 35 and blood sampling was conducted after a week. Newcastle vaccine was injected at day 28 and the antibody titer was determined after two weeks. The highest level of titer of antibody against the SRBC solution was related to the group which received 0.06 ppm nano selenium (p<0.01). These results indicated that nano selenium in comparison with selenium selenite can improve humoral immunity and blood biochemical parameters.

کلیدواژه‌ها [English]

  • Aflatoxin
  • quail
  • selenium
  • humoral immunity
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