مطالعه تاثیر عصاره اتانولی کلم بروکلی بر استرس اکسیداتیو القاء‌شده توسط استامینوفن در کلیه موش صحرایی

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانش‌آموخته دامپزشکی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

2 استاد، گروه پاتوبیولوژی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران

چکیده

مصرف مقادیر زیاد استامینوفن باعث آسیب کلیوی می­شود.هدف از این مطالعه، ارزیابی تعیین اثرات محافظتی عصاره اتانولی کلم بروکلی در مقابل آسیب ایجادشده توسط استامینوفن در بافت­ کلیه موش صحرایی بود. موش‌های صحرایی نر ویستار به­طور تصادفی در 4 گروه 8 سَری توزیع گردیدند. گروه 1 به‌عنوان شاهد، نرمال سالین (ml/kg 10) و گروه 2 عصاره الکلی کلم بروکلی (mg/kg 200) را با تزریق داخل صفاقی به­طور یک­روز در میان، در مدت 14 روز دریافت کردند. گروه 3 استامینوفن را به‌صورت تک­دوز (mg/kg 750) با تزریق داخل صفاقی دریافت کرد. گروه 4 پس از دریافت تک­دوز استامینوفن (mg/kg 750) با تزریق داخل صفاقی، عصاره الکلی کلم بروکلی را مشابه گروه 2 دریافت کرد. در پایان دوره آزمایش، سطح سرمی اوره، اسید اوریک و کراتینین مورد سنجش قرار گرفت. آسیب‌شناسی بافتی نیز جهت ارزیابی درجات مختلف آسیب‌های‌ کلیه انجام گردید. ماحصل پراکسیداسیون لیپیدی (مالون‌دی‌آلدئید)، فعالیت سوپراکسید دیسموتاز، کاتالاز، گلوتاتیون پراکسیداز و گلوتاتیون ردوکتاز جهت ارزیابی استرس اکسیداتیو در هموژنات بافت کلیه مورد سنجش قرار گرفت. عصاره اتانولی کلم بروکلی در موش‌های تیمارشده با استامینوفن به‌طور معنی‌داری مقادیر سرمی اوره، اسید اوریک و کراتینین و میزان پراکسیداسیون لیپیدی بافت کلیه را کاهش و سطوح آنتی‌اکسیدان‌های آن را افزایش داد. در آسیب­شناسی بافتی، عصاره اتانولی کلم بروکلی، جراحات کلیوی موش­ها را بهبود بخشید. یافته‌های بررسی حاضر نشان داد که عصاره اتانولی کلم بروکلی دارای اثرات حفاظتی در برابر سمیّت کلیوی استامینوفن بوده و اثرات محافظتی فوق ممکن است با خواص آنتی‌اکسیدانی و پاک‌سازی رادیکال­های‌ آزاد توسط عصاره مرتبط باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Study on the effects of ethanolic extract of broccoli on oxidative stress induced by acetaminophen in rat kidney

نویسنده [English]

  • Hossein Jeddi 1
1 Geaduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
چکیده [English]

Acetaminophen overdose causes kidney injury. The aim of the present study was to evaluate the protective effect of ethanolic extract of Brassica oleracea L. var. italica against acetaminophen -induced damage in rat kidney. Male Wistar rats were randomly assigned into 4 groups of 8 animals each. Groups I (control) and II received intraperitoneal injections of normal saline (10 ml/kg) and ethanolic extract of broccoli (200 mg/kg) every other day for 14 days. Group Ш received a single intraperitoneal injection of acetaminophen (750 mg/kg). Group IV received ethanolic extract of broccoli similar to group Ш after administration of a single intraperitoneal injection of acetaminophen (750 mg/kg). At the end of experiment, serum levels of urea, uric acid and creatinine were assessed. Moreover, histopathological observations were also conducted to assess the degree of renal tissue injury. Product of lipid peroxidation (malondialdehyde) and activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were assayed in kidney homogenates. In acetaminophen-treated rats, ethanolic extract of broccoli significantly decreased serum urea, uric acid and creatinine and renal tissue peroxidation levels and elevated the values of anti-oxidants. Histopathologically, ethanolic extract of broccoli ameliorated acetaminophen-induced kidney injury. The results of this study indicated that ethanolic extract of broccoli exerts a protective effect against acetaminophen induced renotoxicity in rats which might be related to its anti-oxidant potential.

کلیدواژه‌ها [English]

  • Brassica oleracea L. var. italica
  • Oxidative stress
  • Acetaminophen
  • kidney
  • Rat
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