مطالعه اثر ورزش منظم هوازی بر مقادیر سرمی تروپونین قلبیI ، عامل نکروز توموری آلفا و میزان وقوع آپوپتوز در آسیب ایسکمی-بازخونرسانی قلب موش صحرایی

نوع مقاله: علمی پژوهشی

نویسنده

دانشیار گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

فرآیند بازگرداندن جریان خون به عضله قلب ایسکمیک، می­تواند به­شکل متناقضی موجب القاء آسیب در میوکارد شود. تروپونین قلبی I (cTnI) و عامل نکروز توموری آلفا (TNF-α) شاخص­های بیوشیمیایی مهم آسیب بافت قلب می­باشند. هدف این مطالعه ارزیابی اثرات ورزش هوازی کوتاه­­مدت و درازمدت منظم فزاینده بر مقادیر سرمی cTnI و TNF-α در آسیب ایسکمی-بازخونرسانی (I/R) قلب موش صحرایی می­باشد. بدین منظور، 40 سر موش صحرایی نر ویستار به­طور تصادفی به چهار گروه برابر شامل گروه­های شاهد، I/R، I/R با دو هفته ورزش هوازی­ و I/R با هشت هفته ورزش منظم و فزاینده هوازی­، تقسیم شدند. تمرین هوازی، هفته­ای پنج جلسه دویدن روی تریدمیل با سرعت 25-10 متر/دقیقه به مدت 30-10 دقیقه با شیب 5 درجه انجام شد. برای ایجاد I/R، رگ کرونری چپ پائین­رو توسط کلمپ مسدود و بعد از 30 دقیقه کلمپ­ها باز و به مدت 2 ساعت خونرسانی مجدداً برقرار گردید. در نهایت موش­ها پس از خون­گیری از سینوس پشت کره چشم جهت سنجش cTnI و TNF-α سرم آسان­کُشی شدند. از قلب موش­­ها برش­های بافتی با رنگ­آمیزی تانل تهیه شد. ورزش منظم هوازی درازمدت مقادیر سرمی cTnI و TNF-α را که در اثر I/R افزایش یافته بودند، به­طور معنی­داری (05/0>p) کاهش داد. در آسیب­شناسی بافتی، تعداد سلول­های آپوپتوتیک عضله قلب گروه I/R در مقایسه با گروه شاهد به­طور معنی­داری افزایش یافت (01/0>p). ورزش هوازی منظم و فزاینده درازمدت، تعداد سلول­های آپوپتوتیک را به­طور معنی­داری کاهش داد (05/0>p). نتایج نشان داد ورزش منظم و فزاینده هوازی درازمدت، قلب موش­های صحرایی را از آسیب ایسکمی-خونرسانی مجدد محافظت می­کند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of regular aerobic exercise on serum levels of cTnI, TNF-α and apoptosis in cardiac ischemia-reperfusion injury of the rats

نویسنده [English]

  • y Doustar
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
چکیده [English]

The  process  of  restoring  blood  flow  to  ischemic  heart  muscle  is  antithetically  capable  of
inducing cardiac damage. Cardiac troponin I (cTnI) and tumor necrosis factor alpha (TNF-α) are the important biochemical parameters of cardiac tissue damage. The aim of this study was to evaluate the effects of short term and regular growing long term aerobic exercise on serum levels of cTnI and TNF-α in rats with Ischemia/Reperfusion (I/R) injury. For this purpose, forty male Wistar rats were randomly divided into four equal groups including: control, I/R, I/R with two weeks of aerobic exercise, and I/R with eight weeks of regular growing aerobic exercise groups. Aerobic exercise was performed 5 times per week on treadmill at speed of 10-25m/min for 10-30 minutes with the slope of 5 degrees. For induction of I/R injury, the left descending coronary artery was clamped for 30 minutes, thereafter blood flow was restored for 2 hours. Finally, after collection of blood samples from the retro-orbital plexus for cTnI and TNF-α measurements, all animals were euthanized.  Histologic sections were created for TUNEL staining from the hearts. Regular growing long term aerobic exercise significantly (p<0.05) decreased the cTnI and TNF-α serum levels, which were increased due to I/R injury. Microscopically, the numbers of apoptotic cells were significantly (p<0.01) increased in I/R group compared to the control group. Regular growing long term aerobic exercise significantly decreased the number of apoptotic cells (p<0.05). The results showed regular growing long term aerobic exercise protects the cardiac tissue of rats from I/R injury.
Conflict of interest: None declared.
 

کلیدواژه‌ها [English]

  • HEART
  • Aerobic exercise
  • ischemia/reperfusion
  • cTnI
  • TNF-α
  • Apoptosis
  • Rat
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