بررسی سطح پلاسمایی هپسیدین، سیستاتین سی و ژلسولین در تیلریوز گوسفندی (نژاد قزل) بر اساس سن و شدت پارازیتمی

نوع مقاله: علمی پژوهشی

نویسندگان

1 استادیار، گروه علوم درمانگاهی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران

2 دانش‌آموخته دکترای حرفه ای دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران

چکیده

بیماری تیلریوز یکی از معضلات مهم  صنعت دامداری در اکثر نقاط جهان و ایران بوده که نقش مهمی در کاهش تولید شیر و گوشت داشته و در ضمن باعث ضایعات گسترده خونی و بافتی می‌گردد. هدف از این مطالعه بررسی تغییرات پلاسمایی هپسیدین، سیستاتین سی و ژلسولین در گوسفندان نژاد قزل مبتلا به تیلریوز می‌باشد. در این مطالعه پس از اخذ نمونه خون از گروه‌های بیمار و سالم، اقدام به تعیین فراسنجه­های خونی اعم ازهموگلوبین، هماتوکریت، گلبول­های قرمزو گلبول­های سفید گردید، سپس فراسنجه­ها­ی پلاسمایی فوق‌ نیز با کیت‌های اختصاصی و به کمک تکنیک الایزا اندازه‌گیری شدند. نتایج حاکی از افزایش معنی‌دار(01/0p<)  هپسیدینو سیستاتین سی و کاهش معنی‌دار (01/0p<) ژلسولین در  گروه بیمار (30 رأس) نسبت به گروه سالم (30 رأس) بود. همچنین، افزایش سن و شدت پارازیتمی، باعث تشدید تغییرات شدند، به‌طوری­که بیشترین غلظت هپسیدین و سیستاتین سی و بیشترین تعداد لنفوسیت‌ها در گروه سنی 4 ساله و با شدت پارازیتمی بیش از 4 درصد و کمترین غلظت ژلسولین و سایر فراسنجه­های هماتولوژیک نیز در همان گروه سنی و با همان شدت پارازیتمی مشاهده شد. با توجه به نتایج بدست آمده، به احتمال زیاد التهاب و آسیب سلولی گسترده در گوسفندان مبتلا به تیلریوز در بافت‌های مختلف و به‌خصوص در کلیه­ها وجود دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of plasma hepcidin, cystatin-C and gelsolin in ovine theileriosis (Ghezel breed) based on age and parasitemia rate

نویسندگان [English]

  • Kaveh Azimzadeh 1
  • k Azimzadeh 2
1 Assistant Professor, Department of Clinical Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 Graduate of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
چکیده [English]

The aim of this study was to evaluate alterations of plasma hepcidin, cystatin C and gelsolin in
ovine theileriosis  (Ghezel  breed). Theileriosis is known as an important challenge in the livestock industry in most parts of the world and Iran. It possesses prominent role in reduction of milk and meat production and can induce widespread lesions in blood and tissues. In this study, after blood sampling from healthy sheep and those   affected by theileriosis, blood parameters such as red blood cells, hematocrit, hemoglobin and white blood cells were determined and those plasma parameters were measured by special kits through Elisa technique. The results suggested significant increase (p<0.01) of hepcidin (Hep) and cystatin C and considerable decrease (p<0.01) of gelsolin (Gel) in the infected sheep compared with healthy ones. Also, elevation of age and parasitemia rate, intensified the above mentioned parameters. The highest concentration of hepcidin and cystatin C and largest number of lymphocytes were observed in the 4-year-old group of sheep affected by theileriosis with more than 4 percent parasitemia and the lowest concentration of gelsolin and other hematologic parameters were also observed in the same age group with the same intensity of parasitemia. According to the results, most probably, there are widespread inflammation and cellular damage in various tissues of infected sheep, especially in the kidneys.

کلیدواژه‌ها [English]

  • Sheep theileriosis
  • Hepcidin
  • Cystatin C
  • Gelsolin
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