مطالعه اثر محافظتی ورزش منظم هوازی بر آسیب کلیوی ناشی از مصرف مکمل کراتین منوهیدرات در موش صحرائی

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی ورزشی-تغذیه ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران.

2 گروه فیزیولوژی ورزشی، دانشکده علوم تربیت بدنی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

کراتین یکی از رایج­ترین مکمل‌های‌ بهبودبخش اجرای ورزشی است که توسط ورزشکاران مورد استفاده قرار می‌گیرد. به­علت افزایش بار پروتئینی ناشی از مصرف کراتین، تأثیرات سوء آن بر کلیه به­عنوان مهم­ترین موضوعات قابل بحث وجود داشته است. این مطالعه به منظور ارزیابی اثرات محافظتی ورزش هوازی بر آسیب کلیوی ناشی از مکمل کراتین منوهیدرات در موش صحرائی انجام گرفت. بدین منظور 30 سر موش صحرائی نر ویستار به­طور تصادفی به 3 گروه 10تائی تقسیم گردیدند. گروه اول به عنوان شاهد فقط غذای استاندارد دریافت کرد. گروه دوم روزانه مقدار g/kg b.w. 5 مکمل کراتین منوهیدرات را به مدت 8 هفته به صورت گاواژ و گروه سوم روزانه 30 دقیقه قبل از انجام ورزش هوازی مکمل کراتین منوهیدرات را به همان ترتیب دریافت کردند. تمرین هوازی، هفته­ای پنج جلسه دویدن روی تریدمیل با سرعت 25-10 متر/دقیقه به مدت 30-10 دقیقه با شیب 5 درجه انجام شد. در پایان 8 هفته، میزان آب مصرفی و دفع ادرار موش­ها اندازه­گیری شد و نمونه خون جهت اندازه­گیری شاخص­های سرمی عملکرد کلیه شامل اوره، اسید اوریک و کراتینین اخذ شد. در نهایت موش­ها جهت آسیب­شناسی بافتی کلیه آسان­کُشی شدند. در موش­های گروه 3، با انجام ورزش منظم هوازی، میزان آب مصرفی و دفع ادرار نسبت به گروه 2 به­ طور معنی­داری (05/0>p) افزایش و شاخص­های سرمی آسیب کلیه به­­طور معنی­داری (05/0>p) کاهش پیدا کرد. در گروه 3، مطالعات میکروسکوپی کاهش معنی­دار (05/0>p) آسیب‌های گلومرولی- توبولی و بینابینی کلیه را در مقایسه با گروه 2 نشان داد. نتایج به­دست­آمده نشان داد که انجام ورزش منظم هوازی می­تواند آسیب بافت کلیه را متعاقب دریافت مکمل کراتین منوهیدرات در موش­های صحرایی کاهش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Protective effects of regular aerobic exercise on renal tissue injury following creatine monohydrate supplementation in rats

نویسندگان [English]

  • Davoud Rahimi 1
  • Hassan Matin Homaee 2
چکیده [English]

Creatine is one of the most common supplements for improvement of athletic performance which is used by athletes. The most important debate about creatine consumption is its adverse effect on kidneys due to increased protein load. This study was performed to evaluate the protective effects of aerobic exercise on renal tissue injury following consumption of creatine monohydrate in the rat. For this purpose, 30 male Wistar rats were randomly divided into 3 groups of 10 animals each. Group 1, as control, received only standard food. Group 2 received 5 g/kg b.w. creatine monohydrate supplement daily for 8 weeks through gavage and group 3 received creatine monohydrate supplementation in the same manner30 minutes before aerobic exercise. Aerobic exercise was performed 5 times per week on treadmill at speed of 10-25m/min for 10-30 minutes with the slope of 5 degrees. At the end of 8 weeks, water intake and urinary excretion of rats were measured and blood samples were collected for measurement of serum renal function biomarkers including urea, uric acid and creatinine. Finally, the rats were euthanized for renal histopathology. In group 3, by doing regular aerobic exercise, water intake and urinary excretion rates were significantly (p<0.05) increased compared to group 2 and serum biomarkers of kidney injury decreased significantly (p<0.05). In group 3, microscopic studies showed a significant (p<0.05) reduction of glomerulo-tubular and renal interstitial damages compared to group 2. The results showed that regular aerobic exercise can ameliorate kidney tissue damage following creatine monohydrate supplementation in rats.
Conflict of interest: None declared.

کلیدواژه‌ها [English]

  • Creatine
  • kidney
  • Aerobic exercise
  • Rat
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