بررسی اثرات مکمل اسید اسکوربیک، ویتامین E و بتائین بر عملکرد، پاسخ ایمنی و فراسنجه‌های خونی در جوجه‌های گوشتی

نوع مقاله: علمی پژوهشی

نویسنده

دانشجوی دکترای تخصصی تغذیه دام و طیور، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی - مدرس دانشگاه پیام نور، تهران، ایران

چکیده

به منظور بررسی اثرات ویتامین­های C، E و بتائین بر عملکرد، پاسخ ایمنی و فراسنجه‌های خونی، آزمایشی در قالب طرح کاملاً تصادفی با 6 تیمار و 5 تکرار و 30 قطعه جوجه گوشتی در هر تکرار انجام شد. تیمارهای آزمایشی شامل: شاهد (بدون افزودنی)، اسید اسکوربیک، ویتامین E، بتائین، ویتامین E به­همراه بتائین و اسید اسکوربیک به­همراه ویتامین E و بتائین (افزودنی ها) به جیره پایه بودند. در طول آزمایش صفات وزن، خوراک مصرفی، افزایش وزن، ضریب تبدیل غذایی و تلفات اندازه‌گیری شدند. در سن 35 روزگی جهت بررسی هورمون‌های 3 Tو 4 Tاز هر تکرار دو پرنده انتخاب و از هر پرنده 2 میلی­لیتر خون گرفته شد و پس از جداسازی سرم با استفاده کیت‌های مربوطه میزان هورمون‌های فوق اندازه‌گیری شد. در سن 28 روزگی عیار آنتی­بادی علیه گلبول قرمز گوسفند (SRBC) و همچنین ایمنوگلوبولین‌های G و M مورد بررسی قرار گرفت. وزن زنده، خوراک مصرفی و ضریب تبدیل خوراک در سنین مختلف تحت تأثیر استفاده از افزودنی‌ها قرار نگرفت. تیمار اسید اسکوربیک، ویتامین E و بتائین ماندگاری و شاخص تولید بالاتری نسبت به سایر تیمارها داشت. ولی اختلاف آن با گروه شاهد معنی‌دار نبود و با گروه‌های تغذیه شده با ویتامین E، بتائین و ویتامین E  اختلاف معنی‌داری داشت (05/0>p). عیار آنتی‌بادی علیه گلبول قرمز گوسفند و همچنین ایمنوگلوبولین‌های G وM، درصد هماتوکریت و هورمون­های 3 Tو 4 T نیز تحت تأثیر تیمارهای آزمایشی قرار نگرفت. در نتیجه مکمل‌های مصرفی هیچ تأثیر معنی‌داری بر فاکتورهای مربوط به رشد، فراسنجه‌های خونی و همچنین ایمنی هومورال جوجه های گوشتی نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of supplementing ascorbic acid, vitamin E and betaine on the performance, immune response and blood parameters in broiler

نویسنده [English]

  • S.M Motamedi
Ph.D. Student of Animal and Poultry Nutrition, Department of Animal Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. Lecturer of Payam-e-Noor University, Tehran, Iran.
چکیده [English]

In order to investigate the effects of vitamins C and E and betaine on performance, immune response and blood parameters of broiler chickens, an experiment was conducted with completely randomized block design consisting of 6 treatments and 5 replicates with 30 chicks in each replicate. The groups were assigned to receive the treatment diets as follows: diet with no feed additive (control); diet supplemented with vitamin C; diet supplemented with vitamin E; diet supplemented with betaine; diet supplemented with vitamin E and betaine; diet supplemented with vitamin E, vitamin C and betaine. Body weight, feed intake, body weight gain, feed conversion ratio and mortality were determined over the course of the experiment. At 35 days of age, two birds per replicate were randomly selected and 2 ml blood sample was collected to measure plasma concentration of T3 and T4 hormones. IgM and IgG antibody response to SRBC were also determined at 28 days of age. Body weight, FI and FCR were not affected by different treatments over the course of the study. Broilers fed diet supplemented with vitamin C, vitamin E and betaine showed the highest survival percentage and production index as compared with the other treatments. But it was not significantly different with the control group. IgM and IgG antibody response to SRBC, hematocrit percentage and plasma concentration of T3 and T4 also were not affected by different dietary treatments. The results of this experiment showed that there were no significant differences among the treatments in the performance, immune response and blood parameters of broiler chicken.

کلیدواژه‌ها [English]

  • Ascorbic acid
  • vitamin E
  • betaine
  • Performance
  • immunity
  • blood parameters
  • broiler chicken
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