تاثیر عصاره الکلی فرفیون (Euphorbia cyparissias) بر اسپرماتوژنز و سطح تستوسترون خون در موش صحرایی دیابتی شده با استرپتوزوسین

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه فیزیولوژی، واحد علوم و تحقیقات زنجان، دانشگاه آزاد اسلامی، زنجان، ایران.

2 استادیار گروه فیزیولوژی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

3 گروه فیزیولوژی، مرکز تحقیقات بیولوژی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان، ایران.

چکیده

 دیابت شیرین به­عنوان یک اختلال آندوکرینی مزمن، دارای عوارض جانبی متعدد بوده و یکی از عوامل اصلی مرگ و میر در کشورهای پیشرفته محسوب می‌شود. بیشتر داروهای صناعی مورد استفاده برای مقابله با دیابت و عوارض ناشی از آن در مصرف دراز­مدت دارای اثرات جانبی متعددی هستند. بنابراین، امروزه استفاده از داروهای با منشأ گیاهی به­شدت مورد توجه قرار گرفته است. در طب سنتی از گیاه فرفیون به دلیل اثرات ضدالتهابی، آنتی‌اکسیدانی و ضدتوموری آن استفاده شده است. در این بررسی تعداد 40 سر موش صحرایی نر ویستار در 4 گروه 10 تایی شامل گروه­های شاهد، دیابتی، دیابتی تیمار با عصاره الکلی فرفیون با دز mg/kg250 (تیمار 1) و دیابتی تیمار با عصاره الکلی فرفیون با دز mg/kg500 (تیمار 2) تقسیم شدند. گروه­های تیمار پس از ایجاد دیابت با داروی استرپتوزوسین، عصاره الکلی گیاه فرفیون را با دزهای mg/kg 500 و250 به­مدت 21 روز از طریق گاواژ دریافت کردند. در گروه­های شاهد و دیابتی هم­حجم عصاره، سرم فیزیولوژی گاواژ گردید. در پایان، از گروه­های مختلف نمونه خون جهت اندازه­گیری غلظت سرمی گلوکز و تستوسترون و نمونه بافتی بیضه جهت مطالعه بافت­شناسی و ارزیابی روند اسپرماتوژنز اخذ شد. غلظت سرمی گلوکز و تستوسترون در گروه­ تیمار 2 در مقایسه با گروه­ دیابتی تفاوتی معنی­دار داشت (05/0p

کلیدواژه‌ها


عنوان مقاله [English]

Effects of alcoholic extract of Euphorbia cyparissias on spermatogenesis and serum level of testosterone in streptozotocin-induced diabetic rats

نویسندگان [English]

  • N Mehanfar 1
  • M.A Nourazar 2
  • M Rahnema 3
1 Department of Physiology, Science and Research Branch, Islamic Azad University, Zanjan, Iran.
2 Assistant Professor, Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 Department of Physiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
چکیده [English]

Diabetes mellitus is an endocrine disorder which is considered one of the main causes of mortality in developed countries. Long term use of the majority of synthetic anti-diabetic drugs lead to various side effects therefore the use of medicinal plants has currently become popular. Traditionally, Euphorbia cyparissias has been used for its anti-inflammatory, anti-oxidative and anti-neoplastic properties. In the present study 40 male Wistar rats were studied in four groups (n=10). The groups included: control, diabetic, alcoholic 500, alcoholic 250. The alcoholic groups received 250 and 500 mg/kg of alcoholic extract of Euphorbia cyparissias for 21 days through gastric gavage following induction of diabetes with streptozotocin. In control and diabetes induced groups an identical volume of physiological serum was gavaged. The testicular tissue and blood samples were obtained at the end of the experimental period for histological and blood testosterone concentration purposes, respectively. Results suggested that the serum glucose and testosterone concentrations in the control and alcoholic 500 groups had a significant difference with the other groups (p

کلیدواژه‌ها [English]

  • Euphorbia cyparissias extract
  • Diabetes
  • Testicular tissue
  • Male rat
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